ArticlePloS one2015
Isoproterenol Increases Uncoupling, Glycolysis, and Markers of Beiging in Mature 3T3-L1 Adipocytes.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 56 citations in OpenAlex.
- Impact of METTL3-mediated m6A regulation on early human beige adipogenesis from hiPSCs.Frontiers in cell and developmental biology · 2026Article
- Depot-specific roles for sphingosine kinase 1 in conversion of white adipose to thermogenic adipose.bioRxiv : the preprint server for biology · 2025Article
- Incretin-based approaches for type 2 diabetes therapy: effects on circulating cytokines and adipocyte's secretome.BMC endocrine disorders · 2025Article
- Development of an adipocyte differentiation protocol using 3T3-L1 cells for the investigation of the browning process: identification of the PPAR-γ agonist rosiglitazone as a browning reference drug.Frontiers in pharmacology · 2025Article
- Potential Role of Pig UCP3 in Modulating Adipocyte Browning via the Beta-Adrenergic Receptor Signaling Pathway.Biology · 2024Article
- Mouse vascularized adipose spheroids: an organotypic model for thermogenic adipocytes.Frontiers in endocrinology · 2024Article
- Discovering cellular programs of intrinsic and extrinsic drivers of metabolic traits using LipocyteProfiler.Cell genomics · 2023Article
- The Sympathetic Innervation of Adipose Tissues: Regulation, Functions, and Plasticity.Comprehensive Physiology · 2023Article
- Molecular mechanism of benzo [a] pyrene regulating lipid metabolism via aryl hydrocarbon receptor.Lipids in health and disease · 2022Article
- Evaluating the feasibility of Cas9 overexpression in 3T3-L1 cells for generation of genetic knock-out adipocyte cell lines.Adipocyte · 2021Article
- Review
- Role of Brown and Beige Adipose Tissues in Seasonal Adaptation in the Raccoon Dog (International journal of molecular sciences · 2021Article
- The repertoire of Adhesion G protein-coupled receptors in adipocytes and their functional relevance.International journal of obesity (2005) · 2020Article
- Dynamic changes in DICER levels in adipose tissue control metabolic adaptations to exercise.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- A Peripheral CB1R Antagonist Increases Lipolysis, Oxygen Consumption Rate, and Markers of Beiging in 3T3-L1 Adipocytes Similar to RIM, Suggesting that Central Effects Can Be Avoided.International journal of molecular sciences · 2020Article
- Lipocalin-2 deficiency may predispose to the progression of spontaneous age-related adiposity in mice.Scientific reports · 2020Article
- Transcriptional Regulation of ZNF638 in Thermogenic Cells by the cAMP Response Element Binding Protein in Male Mice.Journal of the Endocrine Society · 2019Article
- Temporal Observation of Adipocyte Microfiber Using Anchoring Device.Micromachines · 2019Article
- Guggulsterone Activates Adipocyte Beiging through Direct Effects on 3T3-L1 Adipocytes and Indirect Effects Mediated through RAW264.7 Macrophages.Medicines (Basel, Switzerland) · 2019Article
- Endoplasmic Reticulum Stress Impaired Uncoupling Protein 1 Expression via the Suppression of Peroxisome Proliferator-Activated Receptor γ Binding Activity in Mice Beige Adipocytes.International journal of molecular sciences · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Beta-adrenergic activation stimulates uncoupling protein 1 (UCP1), enhancing metabolic rate. In vitro, most work has studied brown adipocytes, however, few have investigated more established adipocyte lines such as the murine 3T3-L1 line. To assess the effect of beta-adrenergic activation, mature 3T3-L1s were treated for 6 or 48 hours with or without isoproterenol (10 and 100 μM) following standard differentiation supplemented with thyroid hormone (T3; 1 nM). The highest dose of isoproterenol increased lipid content following 48 hours of treatment. This concentration enhanced UCP1 mRNA and protein expression. The increase in UCP1 following 48 hours of isoproterenol increased oxygen consumption rate. Further, coupling efficiency of the electron transport chain was disturbed and an enhancement of glycolytic rate was measured alongside this, indicating an attempt to meet the energy demands of the cell. Lastly, markers of beige adipocytes (protein content of CD137 and gene transcript of CITED1) were also found to be upregulated at 48 hours of isoproterenol treatment. This data indicates that mature 3T3-L1 adipocytes are responsive to isoproterenol and induce UCP1 expression and activity. Further, this finding provides a model for further pharmaceutical and nutraceutical investigation of UCP1 in 3T3-L1s.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.