Evidence map›Paper›PMID 26389960›Full record

ArticleJournal of functional biomaterials2015

Incorporation of Human Recombinant Tropoelastin into Silk Fibroin Membranes with the View to Repairing Bruch's Membrane.

Audra M A Shadforth, Shuko Suzuki, Raphaelle Alzonne, Grant A Edwards, Neil A Richardson, Traian V Chirila, Damien G Harkin

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of functional biomaterials, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.6field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 15 citations in OpenAlex.

  1. Review
  2. Review
  3. Fibrous Scaffolds From Elastin-Based Materials.Frontiers in bioengineering and biotechnology · 2021
    Review
  4. Article
  5. Article
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Audra M A ShadforthQueensland Eye Institute, 140 Melbourne Street, South Brisbane, Queensland 4101, Australia. audra.shadforth@qei.org.au.
Shuko SuzukiQueensland Eye Institute, 140 Melbourne Street, South Brisbane, Queensland 4101, Australia. shuko.suzuki@qei.org.au.
Raphaelle AlzonneQueensland Eye Institute, 140 Melbourne Street, South Brisbane, Queensland 4101, Australia. raphaelle.alzonne@supersonicimagine.com.
Grant A EdwardsAustralian Institute for Bioengineering and Nanotechnology, University of Queensland, St Lucia, Queensland 4072, Australia. g.edwards1@uq.edu.au.
Neil A RichardsonQueensland Eye Institute, 140 Melbourne Street, South Brisbane, Queensland 4101, Australia. n.richardson@qut.edu.au.
Traian V ChirilaQueensland Eye Institute, 140 Melbourne Street, South Brisbane, Queensland 4101, Australia. g.edwards1@uq.edu.au.
Damien G HarkinQueensland Eye Institute, 140 Melbourne Street, South Brisbane, Queensland 4101, Australia. d.harkin@qut.edu.au.
Queensland Eye Institute · AUQueensland University of Technology · AUUniversity of Queensland · AUUniversity of Western Australia · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bombyx mori silk fibroin membranes provide a potential delivery vehicle for both cells and extracellular matrix (ECM) components into diseased or injured tissues. We have previously demonstrated the feasibility of growing retinal pigment epithelial cells (RPE) on fibroin membranes with the view to repairing the retina of patients afflicted with age-related macular degeneration (AMD). The goal of the present study was to investigate the feasibility of incorporating the ECM component elastin, in the form of human recombinant tropoelastin, into these same membranes. Two basic strategies were explored: (1) membranes prepared from blended solutions of fibroin and tropoelastin; and (2) layered constructs prepared from sequentially cast solutions of fibroin, tropoelastin, and fibroin. Optimal conditions for RPE attachment were achieved using a tropoelastin-fibroin blend ratio of 10 to 90 parts by weight. Retention of tropoelastin within the blend and layered constructs was confirmed by immunolabelling and Fourier-transform infrared spectroscopy (FTIR). In the layered constructs, the bulk of tropoelastin was apparently absorbed into the initially cast fibroin layer. Blend membranes displayed higher elastic modulus, percentage elongation, and tensile strength (p < 0.01) when compared to the layered constructs. RPE cell response to fibroin membranes was not affected by the presence of tropoelastin. These findings support the potential use of fibroin membranes for the co-delivery of RPE cells and tropoelastin.

Indexed as

age-related macular degenerationBombyx moriBruch’s membraneretinal pigment epitheliumsilk fibrointropoelastin

Identifiers

PMID26389960
PMCPMC4598686
OpenAlexW2040082022

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.