ArticleSensors (Basel, Switzerland)2015
A Simple, Low-Cost Platform for Real-Time Isothermal Nucleic Acid Amplification.
Article in Sensors (Basel, Switzerland), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 21 citations in OpenAlex.
- A review of Optical Point-of-Care devices to Estimate the Technology Transfer of These Cutting-Edge Technologies.Biosensors · 2022Review
- Current and Future Perspectives on Isothermal Nucleic Acid Amplification Technologies for Diagnosing Infections.Infection and drug resistance · 2020Review
- Development of a Low-Cost, Wireless Smart Thermostat for Isothermal DNA Amplification in Lab-On-A-Chip Devices.Micromachines · 2019Article
- A portable device for nucleic acid quantification powered by sunlight, a flame or electricity.Nature biomedical engineering · 2018Article
- Point-of-care diagnostic assay for the detection of Zika virus using the recombinase polymerase amplification method.The Journal of general virology · 2018Article
- RPA using a multiplexed cartridge for low cost point of care diagnostics in the field.Analytical biochemistry · 2018Article
- Helicase-dependent isothermal amplification: a novel tool in the development of molecular-based analytical systems for rapid pathogen detection.Analytical and bioanalytical chemistry · 2018Review
- New nucleic acid testing devices to diagnose infectious diseases in resource-limited settings.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2017Review
- Towards Multiplex Molecular Diagnosis-A Review of Microfluidic Genomics Technologies.Micromachines · 2017Review
- An integrated versatile lab-on-a-chip platform for the isolation and nucleic acid-based detection of pathogens.Future science OA · 2017Article
- Future developments in biosensors for field-ready Zika virus diagnostics.Journal of biological engineering · 2017Review
- Functional Metagenomics of Spacecraft Assembly Cleanrooms: Presence of Virulence Factors Associated with Human Pathogens.Frontiers in microbiology · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Advances in microfluidics and the introduction of isothermal nucleic acid amplification assays have resulted in a range of solutions for nucleic acid amplification tests suited for point of care and field use. However, miniaturisation of instrumentation for such assays has not seen such rapid advances and fluorescence based assays still depend on complex, bulky and expensive optics such as fluorescence microscopes, photomultiplier tubes and sensitive lens assemblies. In this work we demonstrate a robust, low cost platform for isothermal nucleic acid amplification on a microfluidic device. Using easily obtainable materials and commercial off-the-shelf components, we show real time fluorescence detection using a low cost photodiode and operational amplifier without need for lenses. Temperature regulation on the device is achieved using a heater fabricated with standard printed circuit board fabrication methods. These facile construction methods allow fabrications at a cost compatible with widespread deployment to resource poor settings.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.