ArticlePloS one2015
ChIP-Seq Analysis of the σE Regulon of Salmonella enterica Serovar Typhimurium Reveals New Genes Implicated in Heat Shock and Oxidative Stress Response.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Article
- Unveiling the novel regulatory roles of RpoD-family sigma factors in Salmonella Typhimurium heat shock response through systems biology approaches.PLoS genetics · 2024Article
- Experimental promoter identification of a foodborne pathogenFrontiers in microbiology · 2023Article
- EbfC/YbaB: A Widely Distributed Nucleoid-Associated Protein in Prokaryotes.Microorganisms · 2022Review
- Gre Factors Are Required for Biofilm Formation inMicroorganisms · 2022Article
- Salmonella Typhimurium outer membrane protein A (OmpA) renders protection from nitrosative stress of macrophages by maintaining the stability of bacterial outer membrane.PLoS pathogens · 2022Article
- RpoE Facilitates Stress-Resistance, Invasion, and Pathogenicity ofMicroorganisms · 2022Article
- Genetic regulation of the ompX porin of Salmonella Typhimurium in response to hydrogen peroxide stress.Biological research · 2022Article
- Insights into the Oxidative Stress Response ofAntioxidants (Basel, Switzerland) · 2020Article
- Involvement of Transcription Elongation Factor GreA inFrontiers in microbiology · 2020Article
- BINDER: computationally inferring a gene regulatory network for Mycobacterium abscessus.BMC bioinformatics · 2019Article
- Salmonella enterica subsp.Applied and environmental microbiology · 2019Article
- Maintaining Integrity Under Stress: Envelope Stress Response Regulation of Pathogenesis in Gram-Negative Bacteria.Frontiers in cellular and infection microbiology · 2019Review
- Article
- Dimethyl adenosine transferase (KsgA) contributes to cell-envelope fitness in Salmonella Enteritidis.Microbiological research · 2018Article
- Transcriptome Analysis ofInternational journal of molecular sciences · 2018Article
- The evolutionary impact of intragenic FliA promoters in proteobacteria.Molecular microbiology · 2018Article
- Novel DNA Binding and Regulatory Activities for σJournal of bacteriology · 2017Article
- Gre factors-mediated control of hilD transcription is essential for the invasion of epithelial cells by Salmonella enterica serovar Typhimurium.PLoS pathogens · 2017Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
The alternative sigma factor σE functions to maintain bacterial homeostasis and membrane integrity in response to extracytoplasmic stress by regulating thousands of genes both directly and indirectly. The transcriptional regulatory network governed by σE in Salmonella and E. coli has been examined using microarray, however a genome-wide analysis of σE-binding sites in Salmonella has not yet been reported. We infected macrophages with Salmonella Typhimurium over a select time course. Using chromatin immunoprecipitation followed by high-throughput DNA sequencing (ChIP-seq), 31 σE-binding sites were identified. Seventeen sites were new, which included outer membrane proteins, a quorum-sensing protein, a cell division factor, and a signal transduction modulator. The consensus sequence identified for σE in vivo binding was similar to the one previously reported, except for a conserved G and A between the -35 and -10 regions. One third of the σE-binding sites did not contain the consensus sequence, suggesting there may be alternative mechanisms by which σE modulates transcription. By dissecting direct and indirect modes of σE-mediated regulation, we found that σE activates gene expression through recognition of both canonical and reversed consensus sequence. New σE regulated genes (greA, luxS, ompA and ompX) are shown to be involved in heat shock and oxidative stress responses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.