Evidence map›Paper›PMID 26389695›Full record

ArticleMolecular endocrinology (Baltimore, Md.)2015

Ajuba Preferentially Binds LXRα/RXRγ Heterodimer to Enhance LXR Target Gene Expression in Liver Cells.

Hongyan Fan, Weibing Dong, Qi Li, Xiuqun Zou, Yihong Zhang, Jiamin Wang, Shengxian Li, Wei Liu, Ying Dong, Haipeng Sun and 1 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular endocrinology (Baltimore, Md.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.5field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. An emerging link between LIM domain proteins and nuclear receptors.Cellular and molecular life sciences : CMLS · 2018
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Hongyan FanDepartment of Endocrinology (H.F., S.L., W.L., Y.D.), Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, 200025 China; Hongqiao Institute of Medicine (H.F., Q.L., X.Z., Y.Z., J.W., Z.H.), Shanghai Tongren Hospital and Faculty of Basic Medicine, Shanghai Jiaotong University School of Medicine, Shanghai, 200025 China; Department of Pathophysiology (W.D., H.S.), Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiaotong University School of Medicine, Shanghai, 200025 China; and Shanghai Key Laboratory for Tumor Microenvironment and Inflammation (Z.H.), Shanghai Jiaotong University School of Medicine, Shanghai, 200025 China.
Weibing DongDepartment of Endocrinology (H.F., S.L., W.L., Y.D.), Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, 200025 China; Hongqiao Institute of Medicine (H.F., Q.L., X.Z., Y.Z., J.W., Z.H.), Shanghai Tongren Hospital and Faculty of Basic Medicine, Shanghai Jiaotong University School of Medicine, Shanghai, 200025 China; Department of Pathophysiology (W.D., H.S.), Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiaotong University School of Medicine, Shanghai, 200025 China; and Shanghai Key Laboratory for Tumor Microenvironment and Inflammation (Z.H.), Shanghai Jiaotong University School of Medicine, Shanghai, 200025 China.
Qi LiDepartment of Endocrinology (H.F., S.L., W.L., Y.D.), Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, 200025 China; Hongqiao Institute of Medicine (H.F., Q.L., X.Z., Y.Z., J.W., Z.H.), Shanghai Tongren Hospital and Faculty of Basic Medicine, Shanghai Jiaotong University School of Medicine, Shanghai, 200025 China; Department of Pathophysiology (W.D., H.S.), Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiaotong University School of Medicine, Shanghai, 200025 China; and Shanghai Key Laboratory for Tumor Microenvironment and Inflammation (Z.H.), Shanghai Jiaotong University School of Medicine, Shanghai, 200025 China.
Xiuqun ZouDepartment of Endocrinology (H.F., S.L., W.L., Y.D.), Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, 200025 China; Hongqiao Institute of Medicine (H.F., Q.L., X.Z., Y.Z., J.W., Z.H.), Shanghai Tongren Hospital and Faculty of Basic Medicine, Shanghai Jiaotong University School of Medicine, Shanghai, 200025 China; Department of Pathophysiology (W.D., H.S.), Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiaotong University School of Medicine, Shanghai, 200025 China; and Shanghai Key Laboratory for Tumor Microenvironment and Inflammation (Z.H.), Shanghai Jiaotong University School of Medicine, Shanghai, 200025 China.
Yihong ZhangDepartment of Endocrinology (H.F., S.L., W.L., Y.D.), Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, 200025 China; Hongqiao Institute of Medicine (H.F., Q.L., X.Z., Y.Z., J.W., Z.H.), Shanghai Tongren Hospital and Faculty of Basic Medicine, Shanghai Jiaotong University School of Medicine, Shanghai, 200025 China; Department of Pathophysiology (W.D., H.S.), Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiaotong University School of Medicine, Shanghai, 200025 China; and Shanghai Key Laboratory for Tumor Microenvironment and Inflammation (Z.H.), Shanghai Jiaotong University School of Medicine, Shanghai, 200025 China.
Jiamin WangDepartment of Endocrinology (H.F., S.L., W.L., Y.D.), Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, 200025 China; Hongqiao Institute of Medicine (H.F., Q.L., X.Z., Y.Z., J.W., Z.H.), Shanghai Tongren Hospital and Faculty of Basic Medicine, Shanghai Jiaotong University School of Medicine, Shanghai, 200025 China; Department of Pathophysiology (W.D., H.S.), Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiaotong University School of Medicine, Shanghai, 200025 China; and Shanghai Key Laboratory for Tumor Microenvironment and Inflammation (Z.H.), Shanghai Jiaotong University School of Medicine, Shanghai, 200025 China.
Shengxian LiDepartment of Endocrinology (H.F., S.L., W.L., Y.D.), Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, 200025 China; Hongqiao Institute of Medicine (H.F., Q.L., X.Z., Y.Z., J.W., Z.H.), Shanghai Tongren Hospital and Faculty of Basic Medicine, Shanghai Jiaotong University School of Medicine, Shanghai, 200025 China; Department of Pathophysiology (W.D., H.S.), Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiaotong University School of Medicine, Shanghai, 200025 China; and Shanghai Key Laboratory for Tumor Microenvironment and Inflammation (Z.H.), Shanghai Jiaotong University School of Medicine, Shanghai, 200025 China.
Wei LiuDepartment of Endocrinology (H.F., S.L., W.L., Y.D.), Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, 200025 China; Hongqiao Institute of Medicine (H.F., Q.L., X.Z., Y.Z., J.W., Z.H.), Shanghai Tongren Hospital and Faculty of Basic Medicine, Shanghai Jiaotong University School of Medicine, Shanghai, 200025 China; Department of Pathophysiology (W.D., H.S.), Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiaotong University School of Medicine, Shanghai, 200025 China; and Shanghai Key Laboratory for Tumor Microenvironment and Inflammation (Z.H.), Shanghai Jiaotong University School of Medicine, Shanghai, 200025 China.
Ying DongDepartment of Endocrinology (H.F., S.L., W.L., Y.D.), Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, 200025 China; Hongqiao Institute of Medicine (H.F., Q.L., X.Z., Y.Z., J.W., Z.H.), Shanghai Tongren Hospital and Faculty of Basic Medicine, Shanghai Jiaotong University School of Medicine, Shanghai, 200025 China; Department of Pathophysiology (W.D., H.S.), Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiaotong University School of Medicine, Shanghai, 200025 China; and Shanghai Key Laboratory for Tumor Microenvironment and Inflammation (Z.H.), Shanghai Jiaotong University School of Medicine, Shanghai, 200025 China.
Haipeng SunDepartment of Endocrinology (H.F., S.L., W.L., Y.D.), Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, 200025 China; Hongqiao Institute of Medicine (H.F., Q.L., X.Z., Y.Z., J.W., Z.H.), Shanghai Tongren Hospital and Faculty of Basic Medicine, Shanghai Jiaotong University School of Medicine, Shanghai, 200025 China; Department of Pathophysiology (W.D., H.S.), Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiaotong University School of Medicine, Shanghai, 200025 China; and Shanghai Key Laboratory for Tumor Microenvironment and Inflammation (Z.H.), Shanghai Jiaotong University School of Medicine, Shanghai, 200025 China.
Zhaoyuan HouDepartment of Endocrinology (H.F., S.L., W.L., Y.D.), Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, 200025 China; Hongqiao Institute of Medicine (H.F., Q.L., X.Z., Y.Z., J.W., Z.H.), Shanghai Tongren Hospital and Faculty of Basic Medicine, Shanghai Jiaotong University School of Medicine, Shanghai, 200025 China; Department of Pathophysiology (W.D., H.S.), Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiaotong University School of Medicine, Shanghai, 200025 China; and Shanghai Key Laboratory for Tumor Microenvironment and Inflammation (Z.H.), Shanghai Jiaotong University School of Medicine, Shanghai, 200025 China.
Renji Hospital · CNTongren Hospital · CNShanghai Jiao Tong University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The liver X receptors (LXRs) are important regulators of lipid, cholesterol, and glucose homeostasis by transcriptional regulation of many key genes in these processes, and the transcriptional activities of LXRs are finely controlled by cooperating with retinoid X receptors and many other coregulators. Here, we report that the LIM protein Ajuba binds to the hinge and the ligand binding domains of LXRα via its C-terminal tandem LIM motifs and enhances LXR target gene expression in liver cells. Depletion of Ajuba in HepG2 cells and in mouse primary hepatocytes decreases LXR target gene expression, whereas stable expression of Ajuba in HepG2 cells results in increased expression of these genes. Mechanistic investigations found that Ajuba selectively interacts with LXRα/retinoid X receptor-γ heterodimer to form a ternary complex, which displays a higher transactivation activity to LXR target genes. Moreover, Ajuba and LXR mutually affect their DNA binding activity at endogenous target chromatins and the cooperation between Ajuba and LXRα is dependent on the functional LXR response elements located in the target promoters. Together, our studies demonstrate that Ajuba is a novel coactivator for LXRs and may play important role in lipid and glucose metabolism.

Indexed as

AnimalsBinding SitesCell LineDNA-Binding ProteinsGene Expression RegulationHEK293 CellsHepatocytesHep G2 CellsHumansLIM Domain ProteinsLiver X ReceptorsMaleMiceMice, Inbred C57BLOrphan Nuclear ReceptorsPromoter Regions, GeneticAJUBA protein, humanDNA-Binding ProteinsLIM Domain ProteinsLiver X ReceptorsNR1H3 protein, humanNr1h3 protein, mouseOrphan Nuclear ReceptorsRetinoid X Receptor gamma

Identifiers

PMID26389695
PMCPMC5414675
OpenAlexW1951880240

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.