ArticleNature methods2015
DNA sense-and-respond protein modules for mammalian cells.
Article in Nature methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Distinct phases of cellular signaling revealed by time-resolved protein synthesis.Nature chemical biology · 2024Article
- A non-viral DNA delivery system consisting of multifunctional chimeric peptide fused with zinc-finger protein.iScience · 2024Article
- Light-switchable transcription factors obtained by direct screening in mammalian cells.Nature communications · 2023Article
- Engineering antiviral immune-like systems for autonomous virus detection and inhibition in mice.Nature communications · 2022Article
- TALE.Sense: A Versatile DNA Sensor Platform for Live Mammalian Cells.ACS synthetic biology · 2022Article
- Programmable protein circuit design.Cell · 2021Review
- A systematic approach to inserting split inteins for Boolean logic gate engineering and basal activity reduction.Nature communications · 2021Article
- Nitroreductase-Mediated Release of Inhibitors of Lysine-Specific Demethylase 1 (LSD1) from Prodrugs in Transfected Acute Myeloid Leukaemia Cells.Chembiochem : a European journal of chemical biology · 2020Article
- Mammalian signaling circuits from bacterial parts.Nature chemical biology · 2020Article
- Artificial signaling in mammalian cells enabled by prokaryotic two-component system.Nature chemical biology · 2020Article
- Rosa26 docking sites for investigating genetic circuit silencing in stem cells.Synthetic biology (Oxford, England) · 2020Article
- Synthetic biology for improving cell fate decisions and tissue engineering outcomes.Emerging topics in life sciences · 2019Article
- Massively parallel RNA device engineering in mammalian cells with RNA-Seq.Nature communications · 2019Article
- Proximity Induced Splicing Utilizing Caged Split Inteins.Journal of the American Chemical Society · 2019Article
- Designing cell function: assembly of synthetic gene circuits for cell biology applications.Nature reviews. Molecular cell biology · 2018Review
- Bottom-up approaches in synthetic biology and biomaterials for tissue engineering applications.Journal of industrial microbiology & biotechnology · 2018Article
- Programming Morphogenesis through Systems and Synthetic Biology.Trends in biotechnology · 2018Review
- Mammalian synthetic biology in the age of genome editing and personalized medicine.Current opinion in chemical biology · 2017Review
- Large-scale design of robust genetic circuits with multiple inputs and outputs for mammalian cells.Nature biotechnology · 2017Article
- Reprogramming the Transcriptional Response to Hypoxia with a Chromosomally Encoded Cyclic Peptide HIF-1 Inhibitor.ACS synthetic biology · 2017Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We generated synthetic protein components that can detect specific DNA sequences and subsequently trigger a desired intracellular response. These modular sensors exploit the programmability of zinc-finger DNA recognition to drive the intein-mediated splicing of an artificial trans-activator that signals to a genetic circuit containing a given reporter or response gene. We used the sensors to mediate sequence recognition-induced apoptosis as well as to detect and report a viral infection. This work establishes a synthetic biology framework for endowing mammalian cells with sentinel capabilities, which provides a programmable means to cull infected cells. It may also be used to identify positively transduced or transfected cells, isolate recipients of intentional genomic edits and increase the repertoire of inducible parts in synthetic biology.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.