ArticleJournal of bone metabolism2015
Discrepancy between Vitamin D Total Immunoassays due to Various Cross-reactivities.
Article in Journal of bone metabolism, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 26 citations in OpenAlex.
- Accre 8 emerging point of care CLIA system for vitamin B12 assessment compared with three established assays.Scientific reports · 2025Article
- An AuNPs-based electrochemical aptasensor for the detection of 25-hydroxy vitamin DAnalytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2024Article
- Vitamin D and Its Metabolites Status before and during Chemotherapy in Caucasian Breast Cancer Patients.Metabolites · 2023Article
- A highly sensitive LC-MS/MS method for quantitative determination of 7 vitamin D metabolites in mouse brain tissue.Analytical and bioanalytical chemistry · 2023Article
- Graphene oxide and fluorescent aptamer based novel biosensor for detection of 25-hydroxyvitamin DScientific reports · 2021Article
- pH assists simultaneous determination of folic acid and vitamin DRSC advances · 2021Article
- Review
- In sickness and in health: pivotal role of vitamin D.Biochemia medica · 2020Review
- Parathyroid hormone reference ranges in healthy individuals classified by vitamin D status.Journal of endocrinological investigation · 2019Article
- Vitamin D Deficiency in the Gulf Cooperation Council: Exploring the Triad of Genetic Predisposition, the Gut Microbiome and the Immune System.Frontiers in immunology · 2019Review
- Concerning the vitamin D reference range: pre-analytical and analytical variability of vitamin D measurement.Biochemia medica · 2017Review
- Vitamin D for skeletal and non-skeletal health: What we should know.Journal of clinical orthopaedics and traumaReview
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe purpose of this study was to find out the cause of discrepancy between various automated immunoassays for 25-hydroxy-vitamin D (25-[OH]D).
methodsNational Institute of Standards & Technology Standard Reference Material (SRM) 972a is SRM for 25-(OH)D and consists of 4 vials of frozen serum with different concentrations of 25-(OH)D. Each concentration was measured 6 times in 3 different immunoassays: ADVIA Vitamin D Total assay (Siemens Healthcare, Erlangen, Germany), ARCHITECT 25-(OH)D (Abbott Laboratories, Abbott Park, IL, USA), and COBAS Vitamin D Total assay (Roche Diagnostics, Basel, Switzerland).
resultsWhen using the certified reference values of SRM 972a as it is, discarding the cross-reactivity of each immunoassay, for ADVIA, the coefficient of determination (R(2)) as a score of regression analysis was 0.8995 and maximal difference between measured value and certified reference value was 3.6 ng/mL in level 3. The R(2) and maximal differences of ARCHITECT were 0.5377 and 6.9 ng/mL, respectively, in level 4. Those of COBAS were 0.3674 and 22.3 ng/mL, respectively, in level 4. When considering cross-reactivities of each immunoassays to various 25-(OH)D metabolites, the ADVIA had R(2) and maximal difference of 0.9254 and 3.3 ng/mL, respectively, in level 3. For ARCHITECT, the R(2) and maximal differences were 0.7602 and 5.1 ng/mL, respectively, in level 1. Those of COBAS were 0.9284 and 4.9 ng/mL, respectively, in level 1.
conclusionsThe cause of discrepancies between vitamin D immunoassays was mainly on the difference in cross-reactivities to various vitamin D metabolites. The discrepancies can be considerably decreased by considering cross-reactivities of each immunoassay.
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