ArticleJournal of biomedical optics2015
Optical properties of mouse brain tissue after optical clearing with FocusClear™.
Article in Journal of biomedical optics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 28 citations in OpenAlex.
- Heat shock protein 40 enhances axon regeneration in a mouse model of traumatic optic neuropathy.Neural regeneration research · 2026Article
- Variation of optical properties of mouse brain using an optical clearing agent: experimental and simulation approaches.Biomedical optics express · 2025Article
- Implantable silicon neural probes with nanophotonic phased arrays for single-lobe beam steering.Communications engineering · 2024Article
- Semi-automated protocol to quantify and characterize fluorescent three-dimensional vascular images.PloS one · 2024Article
- Simple methodology to visualize whole-brain microvasculature in three dimensions.Neurophotonics · 2021Article
- Validation of speed-resolved laser Doppler perfusion in a multimodal optical system using a blood-flow phantom.Journal of biomedical optics · 2019Article
- Optical clearing potential of immersion-based agents applied to thick mouse brain sections.PloS one · 2019Article
- RTF: a rapid and versatile tissue optical clearing method.Scientific reports · 2018Article
- Optic nerve regeneration in the mouse is a complex trait modulated by genetic background.Molecular vision · 2018Article
- Evaluation of a pointwise microcirculation assessment method using liquid and multilayered tissue simulating phantoms.Journal of biomedical optics · 2017Article
- Deep tissue imaging: a review from a preclinical cancer research perspective.Histochemistry and cell biology · 2016Review
- Low-cost tissue simulating phantoms with adjustable wavelength-dependent scattering properties in the visible and infrared ranges.Journal of biomedical optics · 2016Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Fluorescence microscopy is commonly used to investigate disease progression in biological tissues. Biological tissues, however, are strongly scattering in the visible wavelengths, limiting the application of fluorescence microscopy to superficial (<200µm) regions. Optical clearing, which involves incubation of the tissue in a chemical bath, reduces the optical scattering in tissue, resulting in increased tissue transparency and optical imaging depth. The goal of this study was to determine the time- and wavelength-resolved dynamics of the optical scattering properties of rodent brain after optical clearing with FocusClear™. Light transmittance and reflectance of 1-mm mouse brain sections were measured using an integrating sphere before and after optical clearing and the inverse adding doubling algorithm used to determine tissue optical scattering. The degree of optical clearing was quantified by calculating the optical clearing potential (OCP), and the effects of differing OCP were demonstrated using the optical histology method, which combines tissue optical clearing with optical imaging to visualize the microvasculature. We observed increased tissue transparency with longer optical clearing time and an analogous increase in OCP. Furthermore, OCP did not vary substantially between 400 and 1000 nm for increasing optical clearing durations, suggesting that optical histology can improve ex vivo visualization of several fluorescent probes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.