ArticleScientific reports2015
Multiplexed 3D FRET imaging in deep tissue of live embryos.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 24 citations in OpenAlex.
- A cAMP Sensor Based on Ligand-Dependent Protein Stabilization.ACS chemical biology · 2022Article
- Luminescence lifetime imaging of three-dimensional biological objects.Journal of cell science · 2021Article
- Fourier Multiplexed Fluorescence Lifetime Imaging.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Highlights on selected microscopy techniques to study zebrafish developmental biology.Laboratory animal research · 2020Review
- mCerulean3-Based Cameleon Sensor to Explore Mitochondrial CaiScience · 2019Article
- In Vivo Biosensing Using Resonance Energy Transfer.Biosensors · 2019Review
- Dual-FRET imaging of IPScientific reports · 2019Article
- A Genetically Encoded Biosensor Strategy for Quantifying Non-muscle Myosin II Phosphorylation Dynamics in Living Cells and Organisms.Cell reports · 2018Article
- Live-cell imaging: new avenues to investigate retinal regeneration.Neural regeneration research · 2017Review
- Utilizing Zebrafish Visual Behaviors in Drug Screening for Retinal Degeneration.International journal of molecular sciences · 2017Review
- Cellular resolution multiplexed FLIM tomography with dual-color Bessel beam.Biomedical optics express · 2017Article
- Screening for protein-protein interactions using Förster resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM).Scientific reports · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Current deep tissue microscopy techniques are mostly restricted to intensity mapping of fluorophores, which significantly limit their applications in investigating biochemical processes in vivo. We present a deep tissue multiplexed functional imaging method that probes multiple Förster resonant energy transfer (FRET) sensors in live embryos with high spatial resolution. The method simultaneously images fluorescence lifetimes in 3D with multiple excitation lasers. Through quantitative analysis of triple-channel intensity and lifetime images, we demonstrated that Ca(2+) and cAMP levels of live embryos expressing dual FRET sensors can be monitored simultaneously at microscopic resolution. The method is compatible with a broad range of FRET sensors currently available for probing various cellular biochemical functions. It opens the door to imaging complex cellular circuitries in whole live organisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.