ArticleLab on a chip2015
Digital quantification of miRNA directly in plasma using integrated comprehensive droplet digital detection.
Article in Lab on a chip, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 66 citations in OpenAlex.
- Quantitative analysis of miRNAs using SplintR ligase-mediated ligation of complementary-pairing probes enhanced by RNase H (SPLICER)-qPCR.Molecular therapy. Nucleic acids · 2023Article
- Suitability evaluation of toehold switch and EXPAR for cell-free MicroRNA biosensor development.Biotechnology notes (Amsterdam, Netherlands) · 2023Article
- Picoliter Droplet Generation and Dense Bead-in-Droplet Encapsulation via Microfluidic Devices Fabricated via 3D Printed Molds.Micromachines · 2022Article
- Microfluidic circulating reactor system for sensitive and automated duplex-specific nuclease-mediated microRNA detection.Talanta · 2021Article
- Liquid biopsy for therapy monitoring in early-stage non-small cell lung cancer.Molecular cancer · 2021Review
- Extracellular MicroRNAs as Intercellular Mediators and Noninvasive Biomarkers of Cancer.Cancers · 2020Review
- Rapid Detection of β-Lactamase-Producing Bacteria Using the Integrated Comprehensive Droplet Digital Detection (IC 3D) System.Sensors (Basel, Switzerland) · 2020Article
- Droplet microfluidics: fundamentals and its advanced applications.RSC advances · 2020Review
- High-throughput screening by droplet microfluidics: perspective into key challenges and future prospects.Lab on a chip · 2020Article
- Microfluidic systems for rapid antibiotic susceptibility tests (ASTs) at the single-cell level.Chemical science · 2020Review
- Physical and chemical template-blocking strategies in the exponential amplification reaction of circulating microRNAs.Analytical and bioanalytical chemistry · 2020Article
- Rapid bacterial detection and antibiotic susceptibility testing in whole blood using one-step, high throughput blood digital PCR.Lab on a chip · 2020Article
- Isothermal digital detection of microRNAs using background-free molecular circuit.Science advances · 2020Article
- Integrated microfluidic systems with sample preparation and nucleic acid amplification.Lab on a chip · 2019Review
- An ultrasensitive test for profiling circulating tumor DNA using integrated comprehensive droplet digital detection.Lab on a chip · 2019Article
- dPCR: A Technology Review.Sensors (Basel, Switzerland) · 2018Review
- First characterization of a biphasic, switch-like DNA amplification.The Analyst · 2018Article
- Cas9 cleavage assay for pre-screening of sgRNAs using nicking triggered isothermal amplification.Chemical science · 2016Article
- Discovery in Droplets.Analytical chemistry · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 7 institutions in 5 countries.
Funding
Abstract
Quantification of miRNAs in blood can be potentially used for early disease detection, surveillance monitoring and drug response evaluation. However, quantitative and robust measurement of miRNAs in blood is still a major challenge in large part due to their low concentration and complicated sample preparation processes typically required in conventional assays. Here, we present the 'Integrated Comprehensive Droplet Digital Detection' (IC 3D) system where the plasma sample containing target miRNAs is encapsulated into microdroplets, enzymatically amplified and digitally counted using a novel, high-throughput 3D particle counter. Using Let-7a as a target, we demonstrate that IC 3D can specifically quantify target miRNA directly from blood plasma at extremely low concentrations ranging from 10s to 10 000 copies per mL in ≤3 hours without the need for sample processing such as RNA extraction. Using this new tool, we demonstrate that target miRNA content in colon cancer patient blood is significantly higher than that in healthy donor samples. Our IC 3D system has the potential to introduce a new paradigm for rapid, sensitive and specific detection of low-abundance biomarkers in biological samples with minimal sample processing.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.