Evidence map›Paper›PMID 26385614›Full record

ArticleNeuro-oncology2016

Glioma-derived extracellular vesicles selectively suppress immune responses.

Justin E Hellwinkel, Jasmina S Redzic, Tessa A Harland, Dicle Gunaydin, Thomas J Anchordoquy, Michael W Graner

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuro-oncology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.

0numbers the graph read from it
0cells of the map it votes in
80citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

80 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Review
  5. Article
  6. Review
  7. Current Understanding of the Exosomes and Their Associated Biomolecules in the Glioblastoma Biology, Clinical Treatment, and Diagnosis.Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2025
    Review
  8. Review
  9. Article
  10. Article
  11. Specific Binding of Alzheimer's Aβ Peptides to Extracellular Vesicles.International journal of molecular sciences · 2024
    Article
  12. Review
  13. Review
  14. Review
  15. Review
  16. Review
  17. Review
  18. Article
  19. Article
  20. Review

20 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Justin E HellwinkelDept of Neurosurgery, University of Colorado Denver, Anschutz Medical Campus, Aurora, Colorado (J.E.H, T.A.H, D.G., M.W.G); Skaggs School of Pharmacy, University of Colorado Denver, Anschutz Medical Campus, Aurora, Colorado (J.S.R, T.J.A).
Jasmina S RedzicDept of Neurosurgery, University of Colorado Denver, Anschutz Medical Campus, Aurora, Colorado (J.E.H, T.A.H, D.G., M.W.G); Skaggs School of Pharmacy, University of Colorado Denver, Anschutz Medical Campus, Aurora, Colorado (J.S.R, T.J.A).
Tessa A HarlandDept of Neurosurgery, University of Colorado Denver, Anschutz Medical Campus, Aurora, Colorado (J.E.H, T.A.H, D.G., M.W.G); Skaggs School of Pharmacy, University of Colorado Denver, Anschutz Medical Campus, Aurora, Colorado (J.S.R, T.J.A).
Dicle GunaydinDept of Neurosurgery, University of Colorado Denver, Anschutz Medical Campus, Aurora, Colorado (J.E.H, T.A.H, D.G., M.W.G); Skaggs School of Pharmacy, University of Colorado Denver, Anschutz Medical Campus, Aurora, Colorado (J.S.R, T.J.A).
Thomas J AnchordoquyDept of Neurosurgery, University of Colorado Denver, Anschutz Medical Campus, Aurora, Colorado (J.E.H, T.A.H, D.G., M.W.G); Skaggs School of Pharmacy, University of Colorado Denver, Anschutz Medical Campus, Aurora, Colorado (J.S.R, T.J.A).
Michael W GranerDept of Neurosurgery, University of Colorado Denver, Anschutz Medical Campus, Aurora, Colorado (J.E.H, T.A.H, D.G., M.W.G); Skaggs School of Pharmacy, University of Colorado Denver, Anschutz Medical Campus, Aurora, Colorado (J.S.R, T.J.A).

Funding

Colorado Clinical and Translational Sciences InstituteUL1TR001082 · NCATS · UNIVERSITY OF COLORADO DENVER · PI SOKOL, RONALD J. · 2013 to 2017
$48.0M
Tumor-Homing Exosomes for Drug DeliveryR01EB016378 · NIBIB · UNIVERSITY OF COLORADO DENVER · PI ANCHORDOQUY, THOMAS, GRANER, MICHAEL W. · 2013 to 2016
$1.5M
NCATS NIH HHS UL1 TR001082NIBIB NIH HHS 5-R01-EB016378-0NIBIB NIH HHS R01 EB016378
6 · The paper itself

Abstract

backgroundGlioma-related immunosuppression is well documented; however, the mechanisms of suppression are not fully understood. Here we explore a role for glioma extracellular vesicles (EVs) as a means of immune modulation.

methodsHealthy donor peripheral blood mononuclear cells (PBMCs) were incubated with mitogenic stimuli and various concentrations of glioma-derived EVs. Intracellular signaling and cytokine output were determined by protein microarrays, and phenotypic changes were assessed by flow cytometry. Recall antigen testing, mixed lymphocyte reactions, and migration assays analyzed PBMC functional capacity.

resultsProtein microarray data revealed induction of an immunosuppressive phenotype and cytokine output at high tumor-vesicle concentrations but an activated phenotype at low concentrations. T cell activation antigen expression confirmed differential activation profiles. Functional analyses revealed decreased migratory capacity of PBMCs after incubation with EVs; however, recall antigen and mixed lymphocyte tests indicated that activation capacity is still retained in EV-treated cells.

conclusionThe differential effects of high and low EV concentrations dictate modulatory effects on PBMCs. These data provide a role for EVs at high concentrations for inducing selective tolerance of an immune response in a tumor setting. This suggests that lymphocytes in patients' circulation are not irreparably impaired, as previously thought, but can be rescued to augment antitumor responses.

Indexed as

Cells, CulturedCytokinesExosomesExtracellular VesiclesGliomaHumansImmune ToleranceLeukocytes, MononuclearProtein Array AnalysisSignal TransductionT-LymphocytesCytokinesexosomes/extracellular vesiclesgliomaimmune suppressionimmunologyT cells

Identifiers

PMID26385614
PMCPMC4799676

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.