ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2015
Fine genetic mapping of target leaf spot resistance gene cca-3 in cucumber, Cucumis sativus L.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 46 citations in OpenAlex.
- Review
- The Bra1K array: an efficient and versatile 1K liquid-phase SNP chip for genetics and breeding in Chinese cabbage.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Recent progress in DNA marker-assisted selection for vegetable breeding in Japan.Breeding science · 2026Article
- Genome-wide association analysis and KASP markers development for protein quality traits in winter wheat.BMC plant biology · 2025Article
- Analysis of Gene Regulatory Networks of Taro (International journal of genomics · 2025Article
- A genome-wide association study identifies candidate genes for target leaf spot disease resistance in adult cucumber (Frontiers in plant science · 2025Article
- Different control of resistance to two Colletotrichum orbiculare pathogenic races 0 and 1 in cucumber (Cucumis sativus L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Cloning and Functional Analysis of CsROP5 and CsROP10 Genes Involved in Cucumber Resistance toBiology · 2024Article
- Vegetable biology and breeding in the genomics era.Science China. Life sciences · 2023Review
- Deciphering the genetic architecture of resistance toFrontiers in plant science · 2023Article
- Recent status of Genotyping by Sequencing (GBS) Technology in cucumber (Cucumis sativus L.): a review.Molecular biology reports · 2022Review
- Article
- QTL for horticulturally important traits associated with pleiotropic andromonoecy and carpel number loci, and a paracentric inversion in cucumber.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- Recent progress on the molecular breeding of Cucumis sativus L. in China.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Review
- A new SNP genotyping technology Target SNP-seq and its application in genetic analysis of cucumber varieties.Scientific reports · 2020Article
- Molecularly tagged genes and quantitative trait loci in cucumber with recommendations for QTL nomenclature.Horticulture research · 2020Review
- An extended KASP-SNP resource for molecular breeding in Chinese cabbage(Brassica rapa L. ssp. pekinensis).PloS one · 2020Article
- Mildew Resistance Locus O GenesInternational journal of molecular sciences · 2019Article
- CsTFL1 inhibits determinate growth and terminal flower formation through interaction with CsNOT2a in cucumber.Development (Cambridge, England) · 2019Article
- Transcriptome Profile Analysis Reveals thatInternational journal of molecular sciences · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageThe cucumber target leaf spot resistance gene cca - 3 was fine mapped in a 79-kb region harboring a CC-NB-ARC type R gene that may be responsible for the hypersensitive responses to infection of the target leaf spot pathogen in cucumber. The target leaf spot (TLS) is one of the most important foliar diseases in cucumber (Cucumis sativus L.). In this study, we conducted fine genetic mapping of a simply inherited recessive resistance gene, cca-3 against TLS with 193 F2:3 families and 890 F2 plants derived from the resistant cucumber inbred line D31 and the susceptible line D5. Initial mapping with microsatellite markers and bulked segregant analysis placed cca-3 in a 2.5-Mbp region of cucumber chromosome 6. The D5 and D31 lines were re-sequenced at 10× genome coverage to explore new markers in the target region. Genetic mapping in the large F2 population delimited the cca-3 locus in a 79-kb region with flanking markers Indel16874230 and Indel16953846. Additional fine mapping and gene annotation in this region revealed that a CC-NB-ARC type resistance gene analog, Csa6M375730, seems to be the candidate gene for cca-3. One single nucleotide polymorphism (SNP) was found in the NB-ARC domain of this candidate gene sequence between D31 and D5 that may lead to amino acid change, thus altering the function of the conserved NB-ARC motif. This SNP was validated in the segregating population as well as 24 independent cucumber lines. There was significantly higher level of cca-3 expression in the leaves of D5 (susceptible) than in D31 (resistant), and the expression level was positively correlated with the areas of necrotic spots on leaves after inoculation. It seems the cca-3 resistance gene was able to induce hypersensitive responses to the infection by TLS pathogen.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.