ArticleMicrobiology (Reading, England)2015
Motility defects in Campylobacter jejuni defined gene deletion mutants caused by second-site mutations.
Article in Microbiology (Reading, England), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Phylogenetic analyses and antimicrobial resistance profiles of Campylobacter spp. from diarrhoeal patients and chickens in Botswana.PloS one · 2018Trial
- Spatiotemporal variations in the occurrence ofHeliyon · 2024Article
- Identification of Campylobacter jejuni and Campylobacter coli genes contributing to oxidative stress response using TraDIS analysis.BMC microbiology · 2024Article
- Application of TraDIS to define the core essential genome of Campylobacter jejuni and Campylobacter coli.BMC microbiology · 2023Article
- Development and validation of a CRISPR interference system for gene regulation in Campylobacter jejuni.BMC microbiology · 2022Article
- C8J_1298, a bifunctional thiol oxidoreductase of Campylobacter jejuni, affects Dsb (disulfide bond) network functioning.PloS one · 2020Article
- Experimental Evolution ofFrontiers in microbiology · 2020Article
- Genome-Scale Metabolic Model Driven Design of a Defined Medium forFrontiers in microbiology · 2020Article
- CapC, a Novel Autotransporter and Virulence Factor of Campylobacter jejuni.Applied and environmental microbiology · 2018Article
- Antibiotic Sensitivity Profiling and Virulence Potential ofInternational journal of environmental research and public health · 2018Article
- Morphology heterogeneity within a Campylobacter jejuni helical population: the use of calcofluor white to generate rod-shaped C. jejuni 81-176 clones and the genetic determinants responsible for differences in morphology within 11168 strains.Molecular microbiology · 2017Article
- Genome-wide fitness analyses of the foodborne pathogen Campylobacter jejuni in in vitro and in vivo models.Scientific reports · 2017Article
- Analysis of Campylobacter jejuni infection in the gnotobiotic piglet and genome-wide identification of bacterial factors required for infection.Scientific reports · 2017Article
- Identification and initial characterisation of a protein involved in Campylobacter jejuni cell shape.Microbial pathogenesis · 2017Article
- A quantitative proteomic screen of the Campylobacter jejuni flagellar-dependent secretome.Journal of proteomics · 2017Article
- Genomic variations leading to alterations in cell morphology of Campylobacter spp.Scientific reports · 2016Article
- The CsrA-FliW network controls polar localization of the dual-function flagellin mRNA in Campylobacter jejuni.Nature communications · 2016Article
- The Minor Flagellin ofFrontiers in microbiology · 2016Article
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8 authors.
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Abstract
Genetic variation due to mutation and phase variation has a considerable impact on the commensal and pathogenic behaviours of Campylobacter jejuni. In this study, we provide an example of how second-site mutations can interfere with gene function analysis in C. jejuni. Deletion of the flagellin B gene (flaB) in C. jejuni M1 resulted in mutant clones with inconsistent motility phenotypes. From the flaB mutant clones picked for further analysis, two were motile, one showed intermediate motility and two displayed severely attenuated motility. To determine the molecular basis of this differential motility, a genome resequencing approach was used. Second-site mutations were identified in the severely attenuated and intermediate motility flaB mutant clones: a TA-dinucleotide deletion in fliW and an A deletion in flgD, respectively. Restoration of WT fliW, using a newly developed genetic complementation system, confirmed that the second-site fliW mutation caused the motility defect as opposed to the primary deletion of flaB. This study highlights the importance of (i) screening multiple defined gene deletion mutant clones, (ii) genetic complementation of the gene deletion and ideally (iii) screening for second-site mutations that might interfere with the pathways/mechanisms under study.
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