ArticleNucleic acids research2015
The Bacteroides sp. 3_1_23 Pif1 protein is a multifunctional helicase.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 37 citations in OpenAlex.
- Structural mechanism of RECQ1 helicase in unfolding G-quadruplexes compared with duplex DNA.Nucleic acids research · 2025Article
- NAL1 forms a molecular cage to regulate FZP phase separation.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- A high effective expression of human D-glucuronyl C5-epimerase with dimer structure inFrontiers in microbiology · 2025Article
- Eukaryotic Pif1 helicase unwinds G-quadruplex and dsDNA using a conserved wedge.Nature communications · 2024Article
- Structural insights into the N-terminal APHB domain of HrpA: mediating canonical and i-motif recognition.Nucleic acids research · 2024Article
- Tideglusib Inhibits Pif1 Helicase ofACS omega · 2022Article
- Crystal structures of N-terminally truncated telomerase reverse transcriptase from fungi‡.Nucleic acids research · 2021Article
- Structural and functional studies of SF1B Pif1 from Thermus oshimai reveal dimerization-induced helicase inhibition.Nucleic acids research · 2021Article
- Mechanistic Insight Into Cadmium- and Zinc-Induced Inactivation of theFrontiers in molecular biosciences · 2021Article
- The Cellular Functions and Molecular Mechanisms of G-Quadruplex Unwinding Helicases in Humans.Frontiers in molecular biosciences · 2021Review
- Structural basis for DNA unwinding at forked dsDNA by two coordinating Pif1 helicases.Nature communications · 2019Article
- Replication of G Quadruplex DNA.Genes · 2019Review
- Insights into the structural and mechanistic basis of multifunctional S. cerevisiae Pif1p helicase.Nucleic acids research · 2018Article
- The functions of the multi-tasking Pfh1Current genetics · 2017Review
- Mapping and characterization of G-quadruplexes in Mycobacterium tuberculosis gene promoter regions.Scientific reports · 2017Article
- A 3'-5' exonuclease activity embedded in the helicase core domain of Candida albicans Pif1 helicase.Scientific reports · 2017Article
- G-rich telomeric and ribosomal DNA sequences from the fission yeast genome form stable G-quadruplex DNA structures in vitro and are unwound by the Pfh1 DNA helicase.Nucleic acids research · 2016Article
- Mechanistic insight into cadmium-induced inactivation of the Bloom protein.Scientific reports · 2016Article
- Crystal structures of the BsPif1 helicase reveal that a major movement of the 2B SH3 domain is required for DNA unwinding.Nucleic acids research · 2016Article
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
ScPif1 DNA helicase is the prototypical member of a 5'-to-3' helicase superfamily conserved from bacteria to human and plays various roles in the maintenance of genomic homeostasis. While many studies have been performed with eukaryotic Pif1 helicases, including yeast and human Pif1 proteins, the potential functions and biochemical properties of prokaryotic Pif1 helicases remain largely unknown. Here, we report the expression, purification and biochemical analysis of Pif1 helicase from Bacteroides sp. 3_1_23 (BsPif1). BsPif1 binds to a large panel of DNA substrates and, in particular, efficiently unwinds partial duplex DNAs with 5'-overhang, fork-like substrates, D-loop and flap-like substrates, suggesting that BsPif1 may act at stalled DNA replication forks and enhance Okazaki fragment maturation. Like its eukaryotic homologues, BsPif1 resolves R-loop structures and unwinds DNA-RNA hybrids. Furthermore, BsPif1 efficiently unfolds G-quadruplexes and disrupts nucleoprotein complexes. Altogether, these results highlight that prokaryotic Pif1 helicases may resolve common issues that arise during DNA transactions. Interestingly, we found that BsPif1 is different from yeast Pif1, but resembles more human Pif1 with regard to substrate specificity, helicase activity and mode of action. These findings are discussed in the context of the possible functions of prokaryotic Pif1 helicases in vivo.
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