ArticleGenetics2015
Actin Cytoskeletal Organization in Drosophila Germline Ring Canals Depends on Kelch Function in a Cullin-RING E3 Ligase.
Article in Genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Ring canals in the larval adipose of Drosophila buffer stress response.The Journal of cell biology · 2026Article
- Bridges under construction: the dynamics of ring canal expansion during Drosophila oogenesis.Developmental biology · 2026Review
- Cul2 Is Essential for theInternational journal of molecular sciences · 2025Article
- Sequence Variants inMolecular syndromology · 2023Article
- TheiScience · 2022Article
- Precise levels of the Drosophila adaptor protein Dreadlocks maintain the size and stability of germline ring canals.Journal of cell science · 2021Article
- Tissue-specific dynamic codon redefinition inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Topological Data Analysis Approaches to Uncovering the Timing of Ring Structure Onset in Filamentous Networks.Bulletin of mathematical biology · 2021Article
- Suppression of retinal degeneration by two novel ERAD ubiquitin E3 ligases SORDD1/2 in Drosophila.PLoS genetics · 2020Article
- A Novel Kelch-Like-1 Is Involved in Antioxidant Response by Regulating Antioxidant Enzyme System inGenes · 2020Article
- Proximity labeling reveals novel interactomes in liveDevelopment (Cambridge, England) · 2019Article
- Cullin-3 dependent deregulation of ACTN1 represents a new pathogenic mechanism in nemaline myopathy.JCI insight · 2019Article
- Targeted substrate degradation by Kelch controls the actin cytoskeleton during ring canal expansion.Development (Cambridge, England) · 2019Article
- The Misshapen kinase regulates the size and stability of the germline ring canals in the Drosophila egg chamber.Developmental biology · 2018Article
- Cullin 3-Based Ubiquitin Ligases as Master Regulators of Mammalian Cell Differentiation.Trends in biochemical sciences · 2018Review
- Review
- Stay Connected: A Germ Cell Strategy.Trends in genetics : TIG · 2017Review
- A screen for E3 ubiquitination ligases that genetically interact with the adaptor protein Cindr during Drosophila eye patterning.PloS one · 2017Article
- Oncogenic transformation of Drosophila somatic cells induces a functional piRNA pathway.Genes & development · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The Drosophila Kelch protein is required to organize the ovarian ring canal cytoskeleton. Kelch binds and cross-links F-actin in vitro, and it also functions with Cullin 3 (Cul3) as a component of a ubiquitin E3 ligase. How these two activities contribute to cytoskeletal remodeling in vivo is not known. We used targeted mutagenesis to investigate the mechanism of Kelch function. We tested a model in which Cul3-dependent degradation of Kelch is required for its function, but we found no evidence to support this hypothesis. However, we found that mutant Kelch deficient in its ability to interact with Cul3 failed to rescue the kelch cytoskeletal defects, suggesting that ubiquitin ligase activity is the principal activity required in vivo. We also determined that the proteasome is required with Kelch to promote the ordered growth of the ring canal cytoskeleton. These results indicate that Kelch organizes the cytoskeleton in vivo by targeting a protein substrate for degradation by the proteasome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.