ArticleJournal of visualized experiments : JoVE2015
Instillation and Fixation Methods Useful in Mouse Lung Cancer Research.
Article in Journal of visualized experiments : JoVE, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 47 citations in OpenAlex.
- Age-related changes in respiratory system mechanics and lung morphometry.American journal of physiology. Lung cellular and molecular physiology · 2026Article
- A mouse model of lung ischemia-reperfusion injury with reversible left hilar entrapment.Animal models and experimental medicine · 2025Article
- Oropharyngeal Administration of Bleomycin in the Murine Model of Pulmonary Fibrosis.Journal of visualized experiments : JoVE · 2025Article
- A new Col1a1 conditional knock-in mouse model to study osteogenesis imperfecta.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2024Article
- Inhibition of ULK1/2 and KRASeLife · 2024Article
- Argon inhalation attenuates systemic inflammation and rescues lung architecture during experimental neonatal sepsis.Pediatric surgery international · 2023Article
- Curcumin Ameliorates Particulate Matter-Induced Pulmonary Injury through Bimodal Regulation of Macrophage Inflammation via NF-κB and Nrf2.International journal of molecular sciences · 2023Article
- Distinct type I collagen alterations cause intrinsic lung and respiratory defects of variable severity in mouse models of osteogenesis imperfecta.The Journal of physiology · 2023Article
- Loss of E-cadherin is causal to pathologic changes in chronic lung disease.Communications biology · 2022Article
- Impact of E-cig aerosol vaping on fetal and neonatal respiratory development and function.Translational research : the journal of laboratory and clinical medicine · 2022Article
- Reduced SARS-CoV-2 disease outcomes in Syrian hamsters receiving immune sera: Quantitative image analysis in pathologic assessments.Veterinary pathology · 2022Article
- Haploinsufficiency of Col5a1 causes intrinsic lung and respiratory changes in a mouse model of classical Ehlers-Danlos syndrome.Physiological reports · 2022Article
- Mice Dually Disrupted forFrontiers in immunology · 2022Article
- Metformin Alleviates Airway Hyperresponsiveness in a Mouse Model of Diet-Induced Obesity.Frontiers in physiology · 2022Article
- A Method for Orthotopic Transplantation of Lung Cancer in Mice.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Radiofrequency therapy improves exercise capacity of mice with emphysema.Scientific reports · 2021Article
- Downregulation of DUOX1 function contributes to aging-related impairment of innate airway injury responses and accelerated senile emphysema.American journal of physiology. Lung cellular and molecular physiology · 2021Article
- Vitamin D Supplementation: Oxidative Stress Modulation in a Mouse Model of Ovalbumin-Induced Acute Asthmatic Airway Inflammation.International journal of molecular sciences · 2021Article
- A comparison of airway pressures for inflation fixation of developing mouse lungs for stereological analyses.Histochemistry and cell biology · 2021Article
- Second harmonic generation imaging of collagen scaffolds within the alveolar ducts of healthy and emphysematous mouse lungs.Histochemistry and cell biology · 2021Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The ability to instill live agents, cells, or chemicals directly into the lung without injuring or killing the mice is an important tool in lung cancer research. Although there are a number of methods that have been published showing how to intubate mice for pulmonary function measurements, none are without potential problems for rapid tracheal instillation in large cohorts of mice. In the present paper, a simple and quick method is described that enables an investigator to carry out such instillations in an efficient manner. The method does not require any special tools or lighting and can be learned with very little practice. It involves anesthetizing a mouse, making a small incision in the neck to visualize the trachea, and then inserting an intravenous catheter directly. The small incision is quickly closed with tissue adhesive, and the mice are allowed to recover. A skilled student or technician can do instillations at an average rate of 2 min/mouse. Once the cancer is established, there is frequently a need for quantitative histologic analysis of the lungs. Traditionally pathologists usually do not bother to standardize lung inflation during fixation, and analyses are often based on a scoring system that can be quite subjective. While this may sometime be sufficiently adequate for gross estimates of the size of a lung tumor, any proper stereological quantification of lung structure or cells requires a reproducible fixation procedure and subsequent lung volume measurement. Here we describe simple reliable procedures for both fixing the lungs under pressure and then accurately measuring the fixed lung volume. The only requirement is a laboratory balance that is accurate over a range of 1 mg-300 g. The procedures presented here thus could greatly improve the ability to create, treat, and analyze lung cancers in mice.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.