Evidence map›Paper›PMID 26381699›Full record

ArticleScientific reports2015

Dynamic and social behaviors of human pluripotent stem cells.

Smruti M Phadnis, Nathan O Loewke, Ivan K Dimov, Sunil Pai, Christine E Amwake, Olav Solgaard, Thomas M Baer, Bertha Chen, Renee A Reijo Pera

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. An Efficient Protocol for Single-Cell Cloning Human Pluripotent Stem Cells.Frontiers in cell and developmental biology · 2019
    Article
  7. Automated Cell Segmentation for Quantitative Phase Microscopy.IEEE transactions on medical imaging · 2018
    Article
  8. Article
  9. Article
  10. Morphological features of IFN-γ-stimulated mesenchymal stromal cells predict overall immunosuppressive capacity.Proceedings of the National Academy of Sciences of the United States of America · 2017
    Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Smruti M PhadnisInstitute for Stem Cell Biology and Regenerative Medicine, School of Medicine, Stanford University, California, USA.
Nathan O LoewkeDepartment of Electrical Engineering, Stanford University, Stanford, California, USA.
Ivan K DimovInstitute for Stem Cell Biology and Regenerative Medicine, School of Medicine, Stanford University, California, USA.
Sunil PaiInstitute for Stem Cell Biology and Regenerative Medicine, School of Medicine, Stanford University, California, USA.
Christine E AmwakeDepartment of Electrical Engineering, Stanford University, Stanford, California, USA.
Olav SolgaardDepartment of Electrical Engineering, Stanford University, Stanford, California, USA.
Thomas M BaerStanford Photonics Research Center, Department of Applied Physics, Stanford University, Stanford, California, USA.
Bertha ChenDepartment of Obstetrics and Gynecology, School of Medicine, Stanford University, California, USA.
Renee A Reijo PeraInstitute for Stem Cell Biology and Regenerative Medicine, School of Medicine, Stanford University, California, USA.

Funding

Basic and Translational Research iPSC-based hematologic and vascular therapiesU01HL100397 · NHLBI · STANFORD UNIVERSITY · PI COOKE, JOHN P · 2009 to 2015
$7.4M
Stanford University Center for Reproductive and Stem Cell biologyU54HD068158 · NICHD · STANFORD UNIVERSITY · PI FULLER, MARGARET T · 2011 to 2013
$5.0M
Stanford University Center for Reproductive and Stem Cell biologyP50HD068158 · NICHD · STANFORD UNIVERSITY · PI FULLER, MARGARET T · 2014 to 2015
$3.3M
Predoctoral Training in Biomedical Imaging at Stanford UniversityT32EB009653 · NIBIB · STANFORD UNIVERSITY · PI BUTTS-PAULY, KIM · 2010 to 2019
$2.1M
NHLBI NIH HHS U01 HL100397NHLBI NIH HHS U01HL100397NIBIB NIH HHS T32 EB009653NICHD NIH HHS P50 HD068158NICHD NIH HHS P50HD068158NICHD NIH HHS U54 HD068158
6 · The paper itself

Abstract

Human pluripotent stem cells (hPSCs) can self-renew or differentiate to diverse cell types, thus providing a platform for basic and clinical applications. However, pluripotent stem cell populations are heterogeneous and functional properties at the single cell level are poorly documented leading to inefficiencies in differentiation and concerns regarding reproducibility and safety. Here, we use non-invasive time-lapse imaging to continuously examine hPSC maintenance and differentiation and to predict cell viability and fate. We document dynamic behaviors and social interactions that prospectively distinguish hPSC survival, self-renewal, and differentiation. Results highlight the molecular role of E-cadherin not only for cell-cell contact but also for clonal propagation of hPSCs. Results indicate that use of continuous time-lapse imaging can distinguish cellular heterogeneity with respect to pluripotency as well as a subset of karyotypic abnormalities whose dynamic properties were monitored.

Indexed as

BiomarkersCadherinsCell CommunicationCell Culture TechniquesCell DifferentiationCell LineCell Self RenewalCell SurvivalFemaleHumansKaryotypeMalePluripotent Stem CellsTime-Lapse ImagingBiomarkersCadherins

Identifiers

PMID26381699
PMCPMC4585647

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.