ArticleJournal of visualized experiments : JoVE2015
TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos.
Article in Journal of visualized experiments : JoVE, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed.
- Article
- In vivo profiling of the endothelium using 'AngioTag' zebrafish.Angiogenesis · 2025Article
- Arabidopsis as a model for translational research.The Plant cell · 2025Review
- Gap junctions allow transfer of metabolites between germ cells and somatic cells to promote germ cell growth in the Drosophila ovary.PLoS biology · 2025Article
- Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting.Journal of visualized experiments : JoVE · 2021Article
- Dopamine neuron-specific LRRK2 G2019S effects on gene expression revealed by translatome profiling.Neurobiology of disease · 2021Article
- Gelsolin and dCryAB act downstream of muscle identity genes and contribute to preventing muscle splitting and branching in Drosophila.Scientific reports · 2021Article
- Protocol to extract actively translated mRNAs from mouse hypothalamus by translating ribosome affinity purification.STAR protocols · 2021Article
- Identification of a micropeptide and multiple secondary cell genes that modulateProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Trap-TRAP, a Versatile Tool for Tissue-Specific Translatomics in Zebrafish.Frontiers in cell and developmental biology · 2021Article
- Labeling and Isolation of Fluorouracil Tagged RNA by Cytosine Deaminase Expression.Bio-protocol · 2019Article
- Flura-seq identifies organ-specific metabolic adaptations during early metastatic colonization.eLife · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Measuring levels of mRNAs in the process of translation in individual cells provides information on the proteins involved in cellular functions at a given point in time. The protocol dubbed Translating Ribosome Affinity Purification (TRAP) is able to capture this mRNA translation process in a cell-type-specific manner. Based on the affinity purification of polysomes carrying a tagged ribosomal subunit, TRAP can be applied to translatome analyses in individual cells, making it possible to compare cell types during the course of developmental processes or to track disease development progress and the impact of potential therapies at molecular level. Here we report an optimized version of the TRAP protocol, called TRAP-rc (rare cells), dedicated to identifying engaged-in-translation RNAs from rare cell populations. TRAP-rc was validated using the Gal4/UAS targeting system in a restricted population of muscle cells in Drosophila embryos. This novel protocol allows the recovery of cell-type-specific RNA in sufficient quantities for global gene expression analytics such as microarrays or RNA-seq. The robustness of the protocol and the large collections of Gal4 drivers make TRAP-rc a highly versatile approach with potential applications in cell-specific genome-wide studies.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.