ArticleJournal of orthopaedic surgery and research2015
Induction of granulation tissue for the secretion of growth factors and the promotion of bone defect repair.
Article in Journal of orthopaedic surgery and research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 71 citations in OpenAlex.
- Naringin targets TGF-β1-mediated angiogenesis to enhance the osteogenic effect of induced membrane.Bone & joint research · 2026Article
- [Mechanism of actions and reflections of Masquelet induced membrane technique in diabetic wound].Zhonghua shao shang yu chuang mian xiu fu za zhi · 2026Review
- Article
- Induced membrane-mediated DBM in combination with PRP: an autologous bone graft substitute therapy with remarkable osteoformation potency for challenging bone defects.International journal of surgery (London, England) · 2026Article
- Forearm Nonunion and Segmental Bone Defects: A Focused Review of Masquelet Reconstruction and Free Vascularized Bone Grafting.Orthopedic research and reviews · 2026Review
- Optimizing the osteo-immunomodulatory balance: threshold-saturation effects of BMP-2-loaded allografts in the Masquelet's induced membrane technique (MIMT).Frontiers in bioengineering and biotechnology · 2026Article
- Reverse biogradient biomimetic periosteum with osteogenic and angiogenic characteristics for bone regeneration.Materials today. Bio · 2025Article
- Bone wax spacers increase induced membrane thickness and promote osteogenesis more than polymethylmethacrylate spacers in the masquelet technique.European journal of trauma and emergency surgery : official publication of the European Trauma Society · 2025Article
- Osteotropic properties of mesenchymal stromal cells generated in Masquelet's induced membrane technique.Scientific reports · 2025Article
- Paracrine Influence of Masquelet's Induced Membrane on Marrow Derived Mesenchymal Stem Cells.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2025Article
- Mechanisms of the Masquelet technique to promote bone defect repair and its influencing factors.Chinese journal of traumatology = Zhonghua chuang shang za zhi · 2025Review
- Induced Pseudomembrane Enrichment in Long Nerve Allograft Reconstruction.Muscle & nerve · 2025Article
- Engineering the bone reconstruction surgery: the case of the masquelet-induced membrane technique.European journal of trauma and emergency surgery : official publication of the European Trauma Society · 2025Review
- Construction of a 3D Degradable PLLA/β-TCP/CS Scaffold for Establishing an Induced Membrane Inspired by the Modified Single-Stage Masquelet Technique.ACS biomaterials science & engineering · 2025Article
- Masquelet Inspired in Vivo Engineered Extracellular Matrix as Functional Periosteum for Bone Defect Repair and Reconstruction.Advanced healthcare materials · 2025Article
- [Research progress in repair and reconstruction of tumor-related bone defects in proximal femur].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2024Review
- Tibial bone loss.OTA international : the open access journal of orthopaedic trauma · 2024Review
- Characterization of the Masquelet Induced Membrane Technique in a Murine Segmental Bone Defect Model.Revista brasileira de ortopedia · 2023Article
- Management of wounds with exposed bone structures using an induced-membrane followed by polymethyl methacrylate and second-stage skin grafting in the elderly with a 3-year follow-up.International wound journal · 2023Article
- Is the bioactivity of induced membranes time dependent?European journal of trauma and emergency surgery : official publication of the European Trauma Society · 2022Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe use of the Masquelet technique in the repair of large bone defects has gained increased acceptance in recent years. The core of this technique is the induction of granulation tissue membrane formation and the implantation of an autologous cancellous bone to reconstruct bone defects in the membrane. In this study, we purpose to explore the structure of induced membrane and the content of growth factors as well to compare between the structure and the effects on osteogenesis of induced membranes and the periosteum in animal models.
methodsBilateral radial bone defects were generated in 32 healthy adult rabbits. The defects were implanted with bone cement. The induced membranes and periosteum were removed after 2, 4, 6, and 8 weeks. Thereafter, hematoxylin-eosin staining (HE) and an enzyme-linked immunosorbent assay (ELISA) were performed to detect vascular endothelial growth factor (VEGF), angiotensin II (ANG-II), bone morphogenetic protein 2 (BMP2), fibroblast growth factor 2 (FGF2), and prostaglandin E2 (PGE2). Proteins isolated from total cell lysates were cultured with mesenchymal stem cells to test the cell proliferation and alkaline phosphatase activity using epimysium as a control.
resultsThe induced membrane and periosteum exhibited similar structures and growth factor levels after 4 and 6 weeks. The highest concentration of BMP-2 and VEGF in the induced membranes occurred in week 6, and FGF-2 and ANG-II concentrations peaked in week 4. The thickness and vascular density of induced membranes gradually decreased with time.
conclusionInduced membrane matured between the 4th and the 6th week and secreted growth factors to promote osteogenesis. The matured induced membrane and periosteum had similar structures and abilities to promote the osteogenesis of mesenchymal stem cells. However, the induced membrane was thicker than the periosteum.
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