Evidence map›Paper›PMID 26380977›Full record

ArticlePloS one2015

A Novel Detection Platform for Shrimp White Spot Syndrome Virus Using an ICP11-Dependent Immunomagnetic Reduction (IMR) Assay.

Bing-Hsien Liu, Yu-Chen Lin, Chia-Shin Ho, Che-Chuan Yang, Yun-Tsui Chang, Jui-Feng Chang, Chun-Yuan Li, Cheng-Shun Cheng, Jiun-Yan Huang, Yen-Fu Lee and 6 more

Open access · goldAbstract read
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.4field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 11 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 2 institutions in 1 country.

Bing-Hsien LiuMagQu Co., Ltd., New Taipei City 231, Taiwan.
Yu-Chen LinMagQu Co., Ltd., New Taipei City 231, Taiwan.
Chia-Shin HoMagQu Co., Ltd., New Taipei City 231, Taiwan.
Che-Chuan YangMagQu Co., Ltd., New Taipei City 231, Taiwan.
Yun-Tsui ChangMagQu Co., Ltd., New Taipei City 231, Taiwan.
Jui-Feng ChangMagQu Co., Ltd., New Taipei City 231, Taiwan.
Chun-Yuan LiInstitute of Biotechnology, National Cheng Kung University, Tainan 701, Taiwan.
Cheng-Shun ChengInstitute of Biotechnology, National Cheng Kung University, Tainan 701, Taiwan.
Jiun-Yan HuangInstitute of Bioinformatics and Biosignal Transduction, National Cheng Kung University, Tainan 701, Taiwan.
Yen-Fu LeeMagQu Co., Ltd., New Taipei City 231, Taiwan.
Ming-Hung HsuMagQu Co., Ltd., New Taipei City 231, Taiwan.
Feng-Chun LinMagQu Co., Ltd., New Taipei City 231, Taiwan.
Hao-Ching WangGraduate Institute of Translational Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan.
Chu-Fang LoInstitute of Bioinformatics and Biosignal Transduction, National Cheng Kung University, Tainan 701, Taiwan.
Shieh-Yueh YangMagQu Co., Ltd., New Taipei City 231, Taiwan.
Han-Ching WangInstitute of Biotechnology, National Cheng Kung University, Tainan 701, Taiwan.
National Cheng Kung University · TWTaipei Medical University · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Shrimp white spot disease (WSD), which is caused by white spot syndrome virus (WSSV), is one of the world's most serious shrimp diseases. Our objective in this study was to use an immunomagnetic reduction (IMR) assay to develop a highly sensitive, automatic WSSV detection platform targeted against ICP11 (the most highly expressed WSSV protein). After characterizing the magnetic reagents (Fe3O4 magnetic nanoparticles coated with anti ICP11), the detection limit for ICP11 protein using IMR was approximately 2 x 10(-3) ng/ml, and the linear dynamic range of the assay was 0.1~1 x 10(6) ng/ml. In assays of ICP11 protein in pleopod protein lysates from healthy and WSSV-infected shrimp, IMR signals were successfully detected from shrimp with low WSSV genome copy numbers. We concluded that this IMR assay targeting ICP11 has potential for detecting the WSSV.

Indexed as

Magnetite NanoparticlesAnimal DiseasesAnimalsArthropod ProteinsBlotting, WesternImmunoprecipitationLimit of DetectionMagnetic PhenomenaPenaeidaePolymerase Chain ReactionViral Envelope ProteinsWhite spot syndrome virus 1Arthropod ProteinsMagnetite NanoparticlesViral Envelope ProteinsVP28 protein, white spot syndrome virus

Identifiers

PMID26380977
PMCPMC4575139
OpenAlexW2176571465

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.