ArticleSpringerPlus2015
Alkaline thermostable pectinase enzyme from Aspergillus niger strain MCAS2 isolated from Manaslu Conservation Area, Gorkha, Nepal.
Article in SpringerPlus, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 66 citations in OpenAlex.
- Modulation of Maillard reaction pathways and aroma profiles in peanut oil using enzymatically tailored peanut meal hydrolysates.Food chemistry: X · 2026Article
- Study of Different Cultivated Plants Rhizosphere Soil Fungi-Mediated Pectinase: Insights into Production, Optimization, Purification, Biocompatibility, and Application.Microbial ecology · 2025Article
- Immobilization of purified pectinase from Aspergillus nidulans on chitosan and alginate beads for biotechnological applications.Microbial cell factories · 2025Article
- Indigenous fungal strains isolation and molecular identification from coffee pulp for the production of pectic oligosaccharides.3 Biotech · 2023Article
- Fungal pectinases: an insight into production, innovations and applications.World journal of microbiology & biotechnology · 2023Review
- Production, statistical optimization, and functional characterization of alkali stable pectate lyase of Paenibacillus lactis PKC5 for use in juice clarification.Scientific reports · 2022Article
- Partial Purification and Characterization of Exo-Polygalacturonase Produced byLife (Basel, Switzerland) · 2022Article
- Discovery of novel carbohydrate degrading enzymes from soda lakes through functional metagenomics.Frontiers in microbiology · 2022Article
- Wheat Growth Dependent Succession of Culturable Endophytic Bacteria and Their Plant Growth Promoting Traits.Current microbiology · 2021Article
- Thermostable and organic solvent-tolerant acid pectinase from3 Biotech · 2021Article
- Partial Purification and Characterisation of Pectinase Produced byTropical life sciences research · 2021Article
- Screening of pectinase-producing bacteria from farmlands and optimization of enzyme production from selected strain by RSM.Folia microbiologica · 2020Article
- Biochemical Prospects of Various Microbial Pectinase and Pectin: An Approachable Concept in Pharmaceutical Bioprocessing.Frontiers in nutrition · 2020Review
- Cold active pectinase, amylase and protease production by yeast isolates obtained from environmental samples.Extremophiles : life under extreme conditions · 2018Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pectinase enzymes are one of the commercially important enzymes having great potential in various industries especially in food industry. Pectinases accounts for 25 % of global food enzymes produced and their market is increasing day by day. Therefore, the exploration of microorganism with novel characteristics has always been the focus of the research. Microorganism dwelling in unique habitat may possess unique characteristics. As such, a pectinase producing fungus Aspergillus niger strain MCAS2 was isolated from soil of Manaslu Conservation Area (MCA), Gorkha, Nepal. The optimum production of pectinase enzyme was observed at 48 h of fermentation. The pectinase enzyme was partially purified by cold acetone treatment followed by Sephadex G-75 gel filtration chromatography. The partially purified enzyme exhibited maximum activity 60 U/mg which was almost 8.5-fold higher than the crude pectinase. The approximate molecular weight of the enzyme was found to be 66 kDa as observed from SDS-PAGE. The pectinase enzyme was active at broad range of temperature (30-70 °C) and pH (6.2-9.2). Optimum temperature and pH of the pectinase enzyme were 50 °C and 8.2 respectively. The enzyme was stable up to 70 °C and about 82 % of pectinase activity was still observed at 100 °C. The thermostable and alkaline nature of this pectinase can meet the demand of various industrial processes like paper and pulp industry, in textile industry, fruit juice industry, plant tissue maceration and wastewater treatment. In addition, the effect of different metal ions on pectinase activity was also studied.
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