ArticleInternational journal of clinical and experimental medicine2015
Molecular analysis of biofilms on the surface of neonatal endotracheal tubes based on 16S rRNA PCR-DGGE and species-specific PCR.
Article in International journal of clinical and experimental medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Development and internal validation of a nomogram for early prediction of hospital-acquired ESKAPE colonization or infection in very preterm infants using indicators available within 24 hours.Frontiers in pediatrics · 2026Article
- Antimicrobial activity ofPeerJ · 2025Article
- The spectrum of pneumonia among intubated neonates in the neonatal intensive care unit.Journal of perinatology : official journal of the California Perinatal Association · 2024Review
- Green Synthesis of Novel Silver Nanoparticles UsingInternational journal of molecular sciences · 2024Article
- Treatment and Management ofInfection and drug resistance · 2024Review
- Antibacterial and antibiofilm effects of silver nanoparticles against the uropathogenSaudi journal of biological sciences · 2022Article
- Anti-biofilm potential ofSaudi journal of biological sciences · 2022Article
- Autoinducer-2 promotesFrontiers in microbiology · 2022Article
- NO donors and NO delivery methods for controlling biofilms in chronic lung infections.Applied microbiology and biotechnology · 2021Review
- Combined Effect of Naturally-Derived Biofilm Inhibitors and Differentiated HL-60 Cells in the Prevention ofMicroorganisms · 2020Article
- Changes of intestinal microflora in neonatal necrotizing enterocolitis: a single-center study.The Journal of international medical research · 2020Article
- In vivo detection of endotracheal tube biofilms in intubated critical care patients using catheter-based optical coherence tomography.Journal of biophotonics · 2019Article
- Endotracheal Tube Biofilm and its Impact on the Pathogenesis of Ventilator-Associated Pneumonia.Journal of critical care medicine (Universitatea de Medicina si Farmacie din Targu-Mures) · 2018Article
- Effects of Pseudomonas aeruginosa and Streptococcus mitis mixed infection on TLR4-mediated immune response in acute pneumonia mouse model.BMC microbiology · 2017Article
- Autoinducer-2 FacilitatesFrontiers in microbiology · 2017Article
- Autoinducer-2 of Streptococcus mitis as a Target Molecule to Inhibit Pathogenic Multi-Species Biofilm Formation In Vitro and in an Endotracheal Intubation Rat Model.Frontiers in microbiology · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ventilator-associated pneumonia (VAP) results in considerable morbidity and mortality in neonatal intensive care units. VAP is associated with polymicrobial biofilms that form on endotracheal tubes (ETTs). We aimed to evaluate the diversity and the bacterial community in biofilms on ETTs extubated from mechanically ventilated newborns. ETTs (N = 29) and aerobic sputum cultures were obtained from 20 mechanically ventilated newborns. Denaturing gradient gel electrophoresis (DGGE) was used to characterize the bacterial species in the biofilms on the ETTs. Species-specific PCR was used to detect common oropharyngeal Streptococcus species and known ETT-associated pathogens. DGGE profiling of ETT biofilms showed multiple banding patterns indicating a diverse bacterial community. The dominant bacterial species were Klebsiella spp. (29/29), Streptococcus spp. (27/29), and Pseudomonas spp. (24/29). The most frequently occurring Streptococcus species was Streptococcus mitis (N = 18). Oropharyngeal bacteria were present in 25 of 29 ETT specimens. Streptococcus spp. often co-existed with K. pneumoniae and/or P. aeruginosa. In contrast, only one bacterial species was isolated from each sputum culture, K. pneumoniae or Acinetobacter baumannii. Our results demonstrated that Klebsiella spp., Streptococcus spp., and Pseudomonas spp. were the most frequent microbes on the surface of neonatal ETTs. The co-existence of oral commensals and pathogenic bacteria on the same tubes may play a crucial role for biofilm formation.
Indexed as
Identifiers
26379907PMC4565290What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.