Evidence map›Paper›PMID 26379857›Full record

ArticleInternational journal of clinical and experimental medicine2015

The Effect of NRAGE on cell cycle and apoptosis of human dental pulp cells and MDPC-23.

Qi Wu, Shengcai Qi, Ji Ma, Fubo Chen, Jing Chen, Jing Li, Xu Zhang, Yuanzhi Xu, Qiuhui Pan, Raorao Wang

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in International journal of clinical and experimental medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.6field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Complex roles of NRAGE on tumor.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Qi WuDepartment of Clinical Laboratory, Shanghai Tenth People's Hospital, Tongji University Shanghai, China.
Shengcai QiDepartment of Stomatology, Shanghai Tenth People's Hospital, Tongji University Shanghai, China.
Ji MaDepartment of Central Laboratory, Shanghai Tenth People's Hospital, Tongji University Shanghai, China.
Fubo ChenDepartment of Stomatology, Shanghai Tenth People's Hospital, Tongji University Shanghai, China.
Jing ChenDepartment of Clinical Laboratory, Shanghai Tenth People's Hospital, Tongji University Shanghai, China.
Jing LiDepartment of Clinical Laboratory, Shanghai Tenth People's Hospital, Tongji University Shanghai, China.
Xu ZhangDepartment of Stomatology, Shanghai Tenth People's Hospital, Tongji University Shanghai, China.
Yuanzhi XuDepartment of Stomatology, Shanghai Tenth People's Hospital, Tongji University Shanghai, China.
Qiuhui PanDepartment of Central Laboratory, Shanghai Tenth People's Hospital, Tongji University Shanghai, China.
Raorao WangDepartment of Stomatology, Shanghai Tenth People's Hospital, Tongji University Shanghai, China.
Tongji University · CNShanghai Tenth People's Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesNeurotrophin receptor-mediated melanoma antigen-encoding gene homology (NRAGE) is an important regulator of proliferation, cell cycle arrest and apoptosis. Our previous study showed that NRAGE is an important regulator of proliferation and odontogenic differentiation of mouse dental pulp cells. This study aimed to investigate the effects of NRAGE on the cell cycle and apoptosis on human dental pulp cells (hDPCs) and MDPC-23. MATERIALS AND

methodsCells were infected by recombinant lentivirus to stably knockdown the expression of NRAGE, then the biological effects of NRAGE on the MDPC-23 was detected. The cell cycle distributions and apoptosis of hDPCs and MCPC-23 were performed by flow cytometric analysis. Simultaneously, the cell cycle and apoptosis were also detected after cells treated with IKK inhibitor.

resultsThe mRNA and protein levels of NRAGE decreased significantly after infected by recombinant lentivirus. Knockdown of NRAGE inhibited the apoptosis in hDPCs and MCPC-23. Knockdown of NRAGE show significantly G0G1 arrest in hDPCs, while no significantly difference in MDPC-23. Meanwhile, Knockdown of NRAGE activated the NF-κB signaling pathway. After treated with IKK inhibitor, the effect of NRAGE knockdown on apoptosis was reversed in both hDPCs and MDPC-23.

conclusionNRAGE is a potent regulator for cell cycle and apoptosis of hDPCs. Knockdown of NRAGE inhibited apoptosis of hDPCs and MDPC-23 through the NF-κB signaling pathway.

Indexed as

apoptosiscell cyclehuman dental pulp cellsMDPC-23NF-κB signalingNRAGE

Identifiers

PMID26379857
PMCPMC4565240
OpenAlexW2412272167

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.