ArticleInternational journal of clinical and experimental medicine2015
Co-culture of neuroepithelial stem cells with interstitial cells of Cajal results in neuron differentiation.
Article in International journal of clinical and experimental medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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Who cites it
2 citing papers in PubMed, 5 citations in OpenAlex.
- Functional restoration of ex vivo model of pylorus: Co-injection of neural progenitor cells and interstitial cells of Cajal.Stem cells translational medicine · 2020Article
- Development of interstitial cells of Cajal in the human digestive tract as the result of reciprocal induction of mesenchymal and neural crest cells.Journal of cellular and molecular medicine · 2018Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveThe interstitial cells of Cajal (ICCs) interact morphologically and functionally with the elements of the enteric nervous system in the digestive tract. However, direct evidence that ICCs participate in the differentiation of the enteric nervous system is lacking. In this work, we examined in co-culture experiments whether ICCs could stimulate the differentiation of neuroepithelial stem cells (NESCs) to neurons.
methodsNESCs were harvested from the neural tube of embryonic (E11.5) rats, and ICCs were isolated from the colons of newborn rats. Various cell types were identified immunohistochemically.
resultsNESCs reacted with antibodies to the stem-cell marker nest in; ICCs reacted with c-kit antibodies. NESCs, when differentiated into astrocytes, were identified with a marker GFAP, and neurons with marker MAP2. NESCs co-cultured with ICCs, compared with NESCs cultured alone, yielded a significantly greater number of cells positive for the neuronal markers PGP9.5 and nNOS. The co-cultured NESCs also produced more PGP9.5 and nNOS proteins, as measured by Western blotting. In addition, co-cultured ICCs connected morphologically with differentiated NESCs.
conclusionThese in vitro findings demonstrated that ICCs could induce the neuronal differentiation of NESCs, which connected with differentiated neurons into a network morphologically. The findings provide an experimental basis for in vivo application of the simultaneous transplantation of NESCs and ICCs.
Indexed as
Identifiers
26379834PMC4565217W2441865016What OpenQuestion holds
Registered trials
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