ArticleBiotechnology for biofuels2015
Metabolic engineering of Escherichia coli for production of (2S,3S)-butane-2,3-diol from glucose.
Article in Biotechnology for biofuels, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 38 citations in OpenAlex.
- Microbial synthesis of branched-chain β,γ-diols from amino acid metabolism.Nature communications · 2025Article
- Mechanism of microbial production of acetoin and 2,3-butanediol optical isomers and substrate specificity of butanediol dehydrogenase.Microbial cell factories · 2023Review
- Metabolic Engineering and Regulation of Diol Biosynthesis from Renewable Biomass inBiomolecules · 2022Review
- Increasing ATP turnover boosts productivity of 2,3-butanediol synthesis in Escherichia coli.Microbial cell factories · 2021Article
- Dehydrogenation Mechanism of Three Stereoisomers of Butane-2,3-Diol inFrontiers in bioengineering and biotechnology · 2021Article
- Zika Virus Infection Results in Biochemical Changes Associated With RNA Editing, Inflammatory and Antiviral Responses inFrontiers in microbiology · 2020Article
- Shake flask methodology for assessing the influence of the maximum oxygen transfer capacity on 2,3-butanediol production.Microbial cell factories · 2019Article
- Production of optically pure 2,3-butanediol from Miscanthus floridulus hydrolysate using engineered Bacillus licheniformis strains.World journal of microbiology & biotechnology · 2018Article
- Efficient (3S)-Acetoin and (2S,3S)-2,3-Butanediol Production from meso-2,3-Butanediol Using Whole-Cell Biocatalysis.Molecules (Basel, Switzerland) · 2018Article
- Heterologous Overexpression and Biochemical Characterization of a Nitroreductase from Gluconobacter oxydans 621H.Molecular biotechnology · 2016Article
- Mechanism of 2,3-butanediol stereoisomers formation in a newly isolated Serratia sp. T241.Scientific reports · 2016Article
- Editorial: chemicals and bioproducts from biomass.Biotechnology for biofuels · 2016Article
- Engineering Bacillus licheniformis for the production of meso-2,3-butanediol.Biotechnology for biofuels · 2016Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundButane-2,3-diol (2,3-BD) is a fuel and platform biochemical with various industrial applications. 2,3-BD exists in three stereoisomeric forms: (2R,3R)-2,3-BD, meso-2,3-BD and (2S,3S)-2,3-BD. Microbial fermentative processes have been reported for (2R,3R)-2,3-BD and meso-2,3-BD production.
resultsThe production of (2S,3S)-2,3-BD from glucose was acquired by whole cells of recombinant Escherichia coli coexpressing the α-acetolactate synthase and meso-butane-2,3-diol dehydrogenase of Enterobacter cloacae subsp. dissolvens strain SDM. An optimal biocatalyst for (2S,3S)-2,3-BD production, E. coli BL21 (pETDuet-PT7-budB-PT7-budC), was constructed and the bioconversion conditions were optimized. With the addition of 10 mM FeCl3 in the bioconversion system, (2S,3S)-2,3-BD at a concentration of 2.2 g/L was obtained with a stereoisomeric purity of 95.0 % using the metabolically engineered strain from glucose.
conclusionsThe engineered E. coli strain is the first one that can be used in the direct production of (2S,3S)-2,3-BD from glucose. The results demonstrated that the method developed here would be a promising process for efficient (2S,3S)-2,3-BD production.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.