ArticleBiotechnology for biofuels2015
Increasing proline and myo-inositol improves tolerance of Saccharomyces cerevisiae to the mixture of multiple lignocellulose-derived inhibitors.
Article in Biotechnology for biofuels, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 65 citations in OpenAlex.
- Response mechanisms of xylitol-producing Saccharomyces cerevisiae strains to acetic acid and furfural and the role of SPI1, CTT1, and CLB1 in tolerance.Microbial cell factories · 2026Article
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- Overexpression of arginase geneSynthetic and systems biotechnology · 2024Article
- Metabolomic analysis of hydroxycinnamic acid inhibition on Saccharomyces cerevisiae.Applied microbiology and biotechnology · 2024Article
- Review
- General mechanisms of weak acid-tolerance and current strategies for the development of tolerant yeasts.World journal of microbiology & biotechnology · 2023Review
- How adaptive laboratory evolution can boost yeast tolerance to lignocellulosic hydrolyses.Current genetics · 2022Review
- Microbial Adaptation to Enhance Stress Tolerance.Frontiers in microbiology · 2022Review
- Identification of Kic1p and Cdc42p as Novel Targets to Engineer Yeast Acetic Acid Stress Tolerance.Frontiers in bioengineering and biotechnology · 2022Article
- Engineering prokaryotic regulator IrrE to enhance stress tolerance in budding yeast.Biotechnology for biofuels · 2020Article
- Enhanced multi-stress tolerance and glucose utilization of Saccharomyces cerevisiae by overexpression of the SNF1 gene and varied beta isoform of Snf1 dominates in stresses.Microbial cell factories · 2020Article
- Exogenous l-proline improvedBiotechnology for biofuels · 2020Article
- Effect of a Novel Alpha/Beta Hydrolase Domain Protein on Tolerance ofFrontiers in bioengineering and biotechnology · 2020Article
- Effects of MCHM on yeast metabolism.PloS one · 2019Article
- Transcriptomic analysis of thermotolerant yeastRSC advances · 2018Article
- Promiscuous activities of heterologous enzymes lead to unintended metabolic rerouting inBiotechnology for biofuels · 2018Article
- Endogenous lycopene improves ethanol production under acetic acid stress inBiotechnology for biofuels · 2018Article
- Synergistic effect of thioredoxin and its reductase from Kluyveromyces marxianus on enhanced tolerance to multiple lignocellulose-derived inhibitors.Microbial cell factories · 2017Article
- Enhanced fermentative performance under stresses of multiple lignocellulose-derived inhibitors by overexpression of a typical 2-Cys peroxiredoxin fromBiotechnology for biofuels · 2017Article
- Enhanced Bioconversion of Cellobiose by Industrial Saccharomyces cerevisiae Used for Cellulose Utilization.Frontiers in microbiology · 2016Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe development of robust microbes with tolerance to the combined lignocellulose-derived inhibitors is critical for the efficient cellulosic ethanol production. However, the lack of understanding on the inhibition mechanism limited the rational engineering of tolerant strain. Here, through the metabolomic analysis of an adaptation process of Saccharomyces cerevisiae to representative inhibitors, i.e., furfural, acetic acid and phenol (FAP), we figured out the new candidates for improving inhibitor tolerance.
resultsAfter metabolomic analysis, proline and myo-inositol were identified as the potential metabolites responsible for strain tolerance to inhibitors. The deletion of genes involved in proline or myo-inositol synthesis weakened strain tolerance against FAP stress. On the contrary, the addition of proline or myo-inositol in medium exerted a protective effect on cell growth under FAP stress. Furthermore, the enhancement of proline or myo-inositol synthesis by overexpressing key gene PRO1 or INO1 conferred yeast strain significantly increased FAP tolerance. All the recombinant strains finished the fermentation within 60 h under FAP stress, while the control strain was still in the lag phase. Meanwhile, it was found that the intracellular level of reactive oxygen species (ROS) under FAP condition was decreased with the increase of proline content, suggesting the function of proline as a ROS scavenger to protect strains from inhibitor damage.
conclusionIncreasing proline and myo-inositol were uncovered as the new determinants for improving strain tolerance to FAP under the guidance of metabolomics. Meanwhile, this study displayed the powerful application of metabolomics to develop rational strategies to increase stress tolerance and provided valuable insights into the design of recombinant microbes for the complex traits.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.