ArticleBiotechnology for biofuels2015
Physiological roles of pyruvate ferredoxin oxidoreductase and pyruvate formate-lyase in Thermoanaerobacterium saccharolyticum JW/SL-YS485.
Article in Biotechnology for biofuels, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed.
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- Comparative genomics reveals probable adaptations for xylose use in Thermoanaerobacterium saccharolyticum.Extremophiles : life under extreme conditions · 2024Article
- The potential of native and engineered Clostridia for biomass biorefining.Frontiers in bioengineering and biotechnology · 2024Review
- The phenotype and genotype of fermentative prokaryotes.Science advances · 2023Article
- Metabolic engineering of Thermoanaerobacterium AK17 for increased ethanol production in seaweed hydrolysate.Biotechnology for biofuels and bioproducts · 2023Article
- Properties and biotechnological applications of microbial deacetylase.Applied microbiology and biotechnology · 2023Review
- Increasing the Thermodynamic Driving Force of the Phosphofructokinase Reaction inApplied and environmental microbiology · 2022Article
- Characterization ofBiology · 2022Article
- Functional Analysis of HApplied and environmental microbiology · 2022Article
- The pyruvate:ferredoxin oxidoreductase of the thermophilic acetogen, Thermoanaerobacter kivui.FEBS open bio · 2021Article
- Metabolic Fluxes of Nitrogen and Pyrophosphate in Chemostat Cultures of Clostridium thermocellum and Thermoanaerobacterium saccharolyticum.Applied and environmental microbiology · 2020Article
- Development of both type I-B and type II CRISPR/Cas genome editing systems in the cellulolytic bacteriumMetabolic engineering communications · 2020Article
- Conversion of phosphoenolpyruvate to pyruvate inMetabolic engineering communications · 2020Article
- Article
- EngineeredBiotechnology for biofuels · 2019Article
- Metabolic engineering ofBiotechnology for biofuels · 2019Article
- The redox-sensing protein Rex modulates ethanol production in Thermoanaerobacterium saccharolyticum.PloS one · 2018Article
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7 authors.
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Abstract
backgroundThermoanaerobacter saccharolyticum is a thermophilic microorganism that has been engineered to produce ethanol at high titer (30-70 g/L) and greater than 90 % theoretical yield. However, few genes involved in pyruvate to ethanol production pathway have been unambiguously identified. In T. saccharolyticum, the products of six putative pfor gene clusters and one pfl gene may be responsible for the conversion of pyruvate to acetyl-CoA. To gain insights into the physiological roles of PFOR and PFL, we studied the effect of deletions of several genes thought to encode these activities.
resultsIt was found that pyruvate ferredoxin oxidoreductase enzyme (PFOR) is encoded by the pforA gene and plays a key role in pyruvate dissimilation. We further demonstrated that pyruvate formate-lyase activity (PFL) is encoded by the pfl gene. Although the pfl gene is normally expressed at low levels, it is crucial for biosynthesis in T. saccharolyticum. In pforA deletion strains, pfl expression increased and was able to partially compensate for the loss of PFOR activity. Deletion of both pforA and pfl resulted in a strain that required acetate and formate for growth and produced lactate as the primary fermentation product, achieving 88 % theoretical lactate yield.
conclusionPFOR encoded by Tsac_0046 and PFL encoded by Tsac_0628 are only two routes for converting pyruvate to acetyl-CoA in T. saccharolyticum. The physiological role of PFOR is pyruvate dissimilation, whereas that of PFL is supplying C1 units for biosynthesis.
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