ArticleWorld journal of gastroenterology2015
MiR-9a-5p regulates proliferation and migration of hepatic stellate cells under pressure through inhibition of Sirt1.
Article in World journal of gastroenterology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 39 citations in OpenAlex.
- Local fat content determines global and local stiffness in livers with simple steatosis.FASEB bioAdvances · 2023Article
- Human Urine-Derived Stem Cells Improve Partial Bladder Outlet Obstruction in Rats: Preliminary Data and microRNA-mRNA Expression Profile.Stem cell reviews and reports · 2022Article
- Inhibition of Kruppel-like factor 7 attenuates cell proliferation and inflammation of fibroblast-like synoviocytes in rheumatoid arthritis through nuclear factor κB and mitogen-activated protein kinase signaling pathway.Experimental animals · 2022Article
- Overexpression of pressure-responsive miRNA-5703 inhibits pressure-induced growth and metastasis of liver cancer.Journal of Cancer · 2022Article
- MiR-571 affects the development and progression of liver fibrosis by regulating the Notch3 pathway.Scientific reports · 2021Article
- Sirtuins at the Service of Healthy Longevity.Frontiers in physiology · 2021Review
- Inhibited microRNA-494-5p promotes proliferation and suppresses senescence of nucleus pulposus cells in mice with intervertebral disc degeneration by elevating TIMP3.Cell cycle (Georgetown, Tex.) · 2021Article
- MiR-9-5p promotes rabbit preadipocyte differentiation by suppressing leptin gene expression.Lipids in health and disease · 2020Article
- Screening of differentially expressed miRNAs in tensile strain‑treated HepG2 cells by miRNA microarray analysis.Molecular medicine reports · 2020Article
- Changed cellular functions and aberrantly expressed miRNAs and circRNAs in bone marrow stem cells in osteonecrosis of the femoral head.International journal of molecular medicine · 2020Article
- Identification and integrative analysis of microRNAs and mRNAs involved in proliferation and invasion of pressure‑treated human liver cancer cell lines.Molecular medicine reports · 2019Article
- Decline in Sirtuin-1 expression and activity plays a critical role in blood-brain barrier permeability in aging.Neurobiology of disease · 2019Article
- miRNA profiling of urinary exosomes to assess the progression of acute kidney injury.Scientific reports · 2019Article
- Salvianolic Acid A Attenuates Endoplasmic Reticulum Stress and Protects Against Cholestasis-Induced Liver Fibrosis via the SIRT1/HSF1 Pathway.Frontiers in pharmacology · 2018Article
- Effect of shear stress on the migration of hepatic stellate cells.In vitro cellular & developmental biology. Animal · 2018Article
- PU.1-deficient mice are resistant to thioacetamide-induced hepatic fibrosis: PU.1 finely regulates Sirt1 expression via transcriptional promotion ofBioscience reports · 2017Article
- Upregulation of miR-223 in the rat liver inhibits proliferation of hepatocytes under simulated microgravity.Experimental & molecular medicine · 2017Article
- Dysregulation of miRNA Expression in Cancer Associated Fibroblasts (CAFs) and Its Consequences on the Tumor Microenvironment.Cancers · 2017Review
- Hepatic Stellate Cells and microRNAs in Pathogenesis of Liver Fibrosis.Journal of clinical medicine · 2016Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo reveal the functions of microRNAs (miRNAs) with respect to hepatic stellate cells (HSCs) in response to portal hypertension.
methodsPrimary rat HSCs were exposed to static water pressure (10 mmHg, 1 h) and the pressure-induced miRNA expression profile was detected by next-generation sequencing. Quantitative real-time polymerase chain reaction was used to verify the expression of miRNAs. A potential target of MiR-9a-5p was measured by a luciferase reporter assay and Western blot. CCK-8 assay and Transwell assay were used to detect the proliferation and migration of HSCs under pressure.
resultsAccording to the profile, the expression of miR-9a-5p was further confirmed to be significantly increased after pressure overload in HSCs (3.70 ± 0.61 vs 0.97 ± 0.15, P = 0.0226), which resulted in the proliferation, migration and activation of HSCs. In vivo, the up-regulation of miR-9a-5p (2.09 ± 0.91 vs 4.27 ± 1.74, P = 0.0025) and the down-regulation of Sirt1 (2.41 ± 0.51 vs 1.13 ± 0.11, P = 0.0006) were observed in rat fibrotic liver with portal hypertension. Sirt1 was a potential target gene of miR-9a-5p. Through restoring the expression of Sirt1 in miR-9a-5p transfected HSCs on pressure overload, we found that overexpression of Sirt1 could partially abrogate the miR-9a-5p mediated suppression of the proliferation, migration and activation of HSCs.
conclusionOur results suggest that during liver fibrosis, portal hypertension may induce the proliferation, migration and activation of HSCs through the up-regulation of miR-9a-5p, which targets Sirt1.
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