Evidence map›Paper›PMID 26379273›Full record

ArticlePloS one2015

Osteogenic Surface Modification Based on Functionalized Poly-P-Xylylene Coating.

Chih-Hao Chang, Shu-Yun Yeh, Bing-Heng Lee, Chia-Jie Chen, Chiao-Tzu Su, Yen-Ting Lin, Chien-Lin Liu, Hsien-Yeh Chen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Chih-Hao ChangDepartment of Orthopedics, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.
Shu-Yun YehDepartment of Chemical Engineering, National Taiwan University, Taipei, Taiwan.
Bing-Heng LeeDepartment of Orthopedics, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.
Chia-Jie ChenDepartment of Orthopedics, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.
Chiao-Tzu SuDepartment of Chemical Engineering, National Taiwan University, Taipei, Taiwan.
Yen-Ting LinDepartment of Chemical Engineering, National Taiwan University, Taipei, Taiwan.
Chien-Lin LiuDepartment of Orthopaedics and Traumatology, Taipei Veterans General Hospital, Taipei, Taiwan and School of Medicine, National Yang-Ming University, Taipei, Taiwan.
Hsien-Yeh ChenDepartment of Chemical Engineering, National Taiwan University, Taipei, Taiwan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The biotechnology to immobilize biomolecules on material surfaces has been developed vigorously due to its high potentials in medical applications. In this study, a simple and effective method was designed to immobilize biomolecules via amine-N-hydroxysuccinimide (NHS) ester conjugation reaction using functionalized poly-p-xylylene coating on material surfaces. The NHS ester functionalized coating is synthesized via chemical vapor deposition, a facile and solvent-less method, creating a surface which is ready to perform a one-step conjugation reaction. Bone morphogenetic protein 2 (BMP-2) is immobilized onto material surfaces by this coating method, forming an osteogenic environment. The immobilization process is controlled at a low temperature which does not damage proteins. This modified surface induces differentiation of preosteoblast into osteoblast, manifested by alkaline phosphatase (ALP) activity assay, Alizarin Red S (ARS) staining and the expression of osteogenic gene markers, Alpl and Bglap3. With this coating technology, immobilization of growth factors onto material surface can be achieved more simply and more effectively.

Indexed as

3T3 CellsAlkaline PhosphataseAnimalsAnthraquinonesBone Morphogenetic Protein 2Cell AdhesionCell DifferentiationCell LineMiceOsteoblastsOsteogenesisPolymersSuccinimidesSurface PropertiesAlizarin Red SAlkaline PhosphataseALPL protein, mouseAnthraquinonesBmp2 protein, mouseBone Morphogenetic Protein 2N-hydroxysuccinimidePolymersSuccinimides

Identifiers

PMID26379273
PMCPMC4574780

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.