Evidence map›Paper›PMID 26379155›Full record

ArticlePloS one2015

Ultraconserved Elements Sequencing as a Low-Cost Source of Complete Mitochondrial Genomes and Microsatellite Markers in Non-Model Amniotes.

Fábio Raposo do Amaral, Leandro G Neves, Márcio F R Resende, Flávia Mobili, Cristina Y Miyaki, Katia C M Pellegrino, Cibele Biondo

Abstract read
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Fábio Raposo do AmaralUniversidade Federal de São Paulo, Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Laboratório de Genética Evolutiva, Rua Professor Artur Riedel, 275, Diadema, SP, 09972-270, Brazil.
Leandro G NevesRAPiD Genomics, LLC, 747 SW 2nd Avenue, Gainesville, FL, 32601, United States of America.
Márcio F R ResendeRAPiD Genomics, LLC, 747 SW 2nd Avenue, Gainesville, FL, 32601, United States of America.
Flávia MobiliUniversidade Federal de São Paulo, Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Laboratório de Genética Evolutiva, Rua Professor Artur Riedel, 275, Diadema, SP, 09972-270, Brazil.
Cristina Y MiyakiUniversidade de São Paulo, Departamento de Genética e Biologia Evolutiva, Rua do Matão, 277, Cidade Universitária, São Paulo, SP, 05508-090, Brazil.
Katia C M PellegrinoUniversidade Federal de São Paulo, Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Laboratório de Genética Evolutiva, Rua Professor Artur Riedel, 275, Diadema, SP, 09972-270, Brazil.
Cibele BiondoUniversidade Federal do ABC, Centro de Ciências Naturais e Humanas, Rua Arcturus 03, Jardim Antares, São Bernardo do Campo, SP, 09606-070, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Sequence capture of ultraconserved elements (UCEs) associated with massively parallel sequencing has become a common source of nuclear data for studies of animal systematics and phylogeography. However, mitochondrial and microsatellite variation are still commonly used in various kinds of molecular studies, and probably will complement genomic data in years to come. Here we show that besides providing abundant genomic data, UCE sequencing is an excellent source of both sequences for microsatellite loci design and complete mitochondrial genomes with high sequencing depth. Identification of dozens of microsatellite loci and assembly of complete mitogenomes is exemplified here using three species of Poospiza warbling finches from southern and southeastern Brazil. This strategy opens exciting opportunities to simultaneously analyze genome-wide nuclear datasets and traditionally used mtDNA and microsatellite markers in non-model amniotes at no additional cost.

Indexed as

AnimalsBrazilDNA, MitochondrialFinchesGenetic MarkersGenome, MitochondrialMicrosatellite RepeatsMolecular Sequence DataPhylogenySequence Analysis, DNADNA, MitochondrialGenetic Markers

Identifiers

PMID26379155
PMCPMC4574942

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.