ArticleInternational journal of molecular sciences2015
Molecular Identification of Dendrobium Species (Orchidaceae) Based on the DNA Barcode ITS2 Region and Its Application for Phylogenetic Study.
Article in International journal of molecular sciences, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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33 citing papers in PubMed.
- Integrated ITS2 DNA Barcoding and LC-QTOF/MS Metabolomics for Authentication of Ngueak Pla Mo (Plants (Basel, Switzerland) · 2026Article
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- UntanglingGenes · 2025Article
- Chloroplast genome, an effective strategy for identifying hybrid species, using Dendrobium 'Black Gold' as an example.BMC plant biology · 2025Article
- Evolution of morphological traits of Dendrobium sensu lato (Orchidaceae)-an attempt to resolve phylogenetic relationships in nominal and morphologically convergent sections.BMC plant biology · 2025Article
- Unlocking the Genetic Identity of EndangeredGenes · 2024Article
- Single nucleotide polymorphism-based visual identification ofFrontiers in plant science · 2024Article
- Feasibility study on the introduction of Micro-CT technology for the identification ofFrontiers in pharmacology · 2024Article
- Identification of the Plant Family Caryophyllaceae in Korea Using DNA Barcoding.Plants (Basel, Switzerland) · 2023Article
- Evolution patterns of NBS genes in the genus Dendrobium and NBS-LRR gene expression in D. officinale by salicylic acid treatment.BMC plant biology · 2022Article
- Orchidaceae-Derived Anticancer Agents: A Review.Cancers · 2022Review
- Structural characterization of Platanthera ussuriensis chloroplast genome and comparative analyses with other species of Orchidaceae.BMC genomics · 2022Article
- The chromosome-level reference genome assembly forActa pharmaceutica Sinica. B · 2021Article
- The specific DNA barcodes based on chloroplast genes for species identification of Orchidaceae plants.Scientific reports · 2021Article
- Development, Identification, and Application of a Germplasm Specific SCAR Marker forFrontiers in plant science · 2021Article
- The low copy nuclear region, RPB2 as a novel DNA barcode region for species identification in the rattan genusPhysiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Article
- Recent advances of novel technologies for quality consistency assessment of natural herbal medicines and preparations.Chinese medicine · 2020Review
- Genetic Diversity and Identification of VietnameseBiology · 2019Article
- Molecular authentication and differentiation of Dendrobium species by rDNA ITS region sequence analysis.AMB Express · 2019Article
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7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The over-collection and habitat destruction of natural Dendrobium populations for their commercial medicinal value has led to these plants being under severe threat of extinction. In addition, many Dendrobium plants are similarly shaped and easily confused during the absence of flowering stages. In the present study, we examined the application of the ITS2 region in barcoding and phylogenetic analyses of Dendrobium species (Orchidaceae). For barcoding, ITS2 regions of 43 samples in Dendrobium were amplified. In combination with sequences from GenBank, the sequences were aligned using Clustal W and genetic distances were computed using MEGA V5.1. The success rate of PCR amplification and sequencing was 100%. There was a significant divergence between the inter- and intra-specific genetic distances of ITS2 regions, while the presence of a barcoding gap was obvious. Based on the BLAST1, nearest distance and TaxonGAP methods, our results showed that the ITS2 regions could successfully identify the species of most Dendrobium samples examined; Second, we used ITS2 as a DNA marker to infer phylogenetic relationships of 64 Dendrobium species. The results showed that cluster analysis using the ITS2 region mainly supported the relationship between the species of Dendrobium established by traditional morphological methods and many previous molecular analyses. To sum up, the ITS2 region can not only be used as an efficient barcode to identify Dendrobium species, but also has the potential to contribute to the phylogenetic analysis of the genus Dendrobium.
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