ArticleMolecular biology of the cell2015
Conserved function of the lysine-based KXD/E motif in Golgi retention for endomembrane proteins among different organisms.
Article in Molecular biology of the cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 48 citations in OpenAlex.
- Siamese Twins: The Multimodular Mechanisms of Golgi Maturation and Glycan Synthesis Are Coupled at Their Core.Sub-cellular biochemistry · 2026Review
- Novel regulators of heparan sulfate proteoglycans modulate cellular uptake of α-synuclein fibrils.Communications biology · 2025Article
- COPI-dependent intra-Golgi recycling at an intermediate stage of cisternal maturation.bioRxiv : the preprint server for biology · 2025Article
- An Overview of Protein Secretion in Plant Cells.Methods in molecular biology (Clifton, N.J.) · 2024Review
- The presence of β'1-COP and β'2-COP is required for female and male gametophyte development.Plant reproduction · 2023Article
- VAMP724 and VAMP726 are involved in autophagosome formation inAutophagy · 2023Article
- Differential Involvement ofCells · 2022Article
- Critical Determinants in ER-Golgi Trafficking of Enzymes Involved in Glycosylation.Plants (Basel, Switzerland) · 2022Review
- Molecular mechanisms of endomembrane trafficking in plants.The Plant cell · 2022Review
- An Update on the Key Factors Required for Plant Golgi Structure Maintenance.Frontiers in plant science · 2022Review
- Role of theFrontiers in cell and developmental biology · 2022Review
- Normal oil body formation inFrontiers in plant science · 2022Article
- Motif-based endomembrane trafficking.Plant physiology · 2021Article
- ß-COP mutants show specific high sensitivity to chloride ions.Plant signaling & behavior · 2021Article
- Conserved C-terminal motifs in odorant receptors instruct their cell surface expression and cAMP signaling.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Organelle Visualization With Multicolored Fluorescent Markers in Bamboo.Frontiers in plant science · 2021Article
- COPI complex isoforms are required for the early acceptance of compatible pollen grains in Arabidopsis thaliana.Plant reproduction · 2020Article
- Loss ofFrontiers in plant science · 2020Article
- Separating Golgi Proteins fromThe Plant cell · 2019Article
- The Golgi Localization of GnTI Requires a Polar Amino Acid Residue within Its Transmembrane Domain.Plant physiology · 2019Article
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We recently identified a new COPI-interacting KXD/E motif in the C-terminal cytosolic tail (CT) of Arabidopsis endomembrane protein 12 (AtEMP12) as being a crucial Golgi retention mechanism for AtEMP12. This KXD/E motif is conserved in CTs of all EMPs found in plants, yeast, and humans and is also present in hundreds of other membrane proteins. Here, by cloning selective EMP isoforms from plants, yeast, and mammals, we study the localizations of EMPs in different expression systems, since there are contradictory reports on the localizations of EMPs. We show that the N-terminal and C-terminal GFP-tagged EMP fusions are localized to Golgi and post-Golgi compartments, respectively, in plant, yeast, and mammalian cells. In vitro pull-down assay further proves the interaction of the KXD/E motif with COPI coatomer in yeast. COPI loss of function in yeast and plants causes mislocalization of EMPs or KXD/E motif-containing proteins to vacuole. Ultrastructural studies further show that RNA interference (RNAi) knockdown of coatomer expression in transgenic Arabidopsis plants causes severe morphological changes in the Golgi. Taken together, our results demonstrate that N-terminal GFP fusions reflect the real localization of EMPs, and KXD/E is a conserved motif in COPI interaction and Golgi retention in eukaryotes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.