ArticleThe Journal of biological chemistry2015
20-Hydroxyecdysone (20E) Primary Response Gene E93 Modulates 20E Signaling to Promote Bombyx Larval-Pupal Metamorphosis.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 125 citations in OpenAlex.
- A self-attention-based deep learning model for identifying key genes in insect pupal metamorphosis.BMC genomics · 2026Article
- Rewiring of two conserved signalling hubs into one axis underlies convergent female-specific wing regression in insects.Nature ecology & evolution · 2026Article
- Identification, expression profiling, and functional characterization of trehalase genes (OaTRE1 and OaTRE) in Oedaleus asiaticus (Orthoptera: Acridoidea).Journal of insect science (Online) · 2026Article
- Characterization of the Crustacean Methyl Farnesoate Transcriptional Signaling Genes.International journal of molecular sciences · 2026Article
- Histone acetylation homeodynamics navigates cell survival and apoptosis.Nature communications · 2025Article
- Genomic and insulin-mediated control of metabolic homeostasis by the mosquito ecdysone-induced gene E93.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Effects of Novaluron Exposure on the Oviposition and Expression of Ovarian Development Related Genes in Silkworm,Insects · 2024Article
- E93 controls adult differentiation by repressingProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Replacement of fish meal with full fatHeliyon · 2024Article
- Methoprene-Tolerant (Met) Acts as Methyl Farnesoate Receptor to Regulate Larva Metamorphosis in Mud Crab,International journal of molecular sciences · 2024Article
- 5mC modification orchestrates choriogenesis and fertilization by preventing prolonged ftz-f1 expression.Nature communications · 2023Article
- Research Progress on the Regulation of Autophagy and Apoptosis in Insects by Sterol Hormone 20-Hydroxyecdysone.Insects · 2023Review
- Overexpression ofInternational journal of molecular sciences · 2023Article
- Sterol Regulation of Development and 20-Hydroxyecdysone Biosynthetic and Signaling Genes inCells · 2023Article
- Adenosine Monophosphate-Activated Protein Kinase (AMPK) Phosphorylation Is Required for 20-Hydroxyecdysone Regulates Ecdysis inInternational journal of molecular sciences · 2023Article
- Autophagy genes AMBRA1 and ATG8 play key roles in midgut remodeling of the yellow fever mosquito,Frontiers in insect science · 2023Article
- A critical role for ecdysone response genes in regulating egg production in adult female Rhodnius prolixus.PloS one · 2023Article
- Mblk-1/E93, an ecdysone related-transcription factor, targets synaptic plasticity-related genes in the honey bee mushroom bodies.Scientific reports · 2022Article
- CRISPR-Cas9 Genome Editing Uncovers the Mode of Action of Methoprene in the Yellow Fever Mosquito,The CRISPR journal · 2022Article
Corrections and comments
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Authors and funding
10 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As revealed in a previous microarray study to identify genes regulated by 20-hydroxyecdysone (20E) and juvenile hormone (JH) in the silkworm, Bombyx mori, E93 expression in the fat body was markedly low prior to the wandering stage but abundant during larval-pupal metamorphosis. Induced by 20E and suppressed by JH, E93 expression follows this developmental profile in multiple silkworm alleles. The reduction of E93 expression by RNAi disrupted 20E signaling and the 20E-induced autophagy, caspase activity, and cell dissociation in the fat body. Reducing E93 expression also decreased the expression of the 20E-induced pupal-specific cuticle protein genes and prevented growth and differentiation of the wing discs. Importantly, the two HTH domains in E93 are critical for inducing the expression of a subset of 20E response genes, including EcR, USP, E74, Br-C, and Atg1. By contrast, the LLQHLL and PLDLSAK motifs in E93 inhibit its transcriptional activity. E93 binds to the EcR-USP complex via a physical association with USP through its LLQHLL motif; and this association is enhanced by 20E-induced EcR-USP interaction, which attenuates the transcriptional activity of E93. E93 acts through the two HTH domains to bind to GAGA-containing motifs present in the Atg1 promoter region for inducing gene expression. In conclusion, E93 transcriptionally modulates 20E signaling to promote Bombyx larval-pupal metamorphosis.
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