ArticleMolecular genetics and genomics : MGG2016
Comparative transcriptome analyses of deltamethrin-susceptible and -resistant Culex pipiens pallens by RNA-seq.
Article in Molecular genetics and genomics : MGG, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 61 citations in OpenAlex.
- Integrated transcriptome-microbiome analysis reveals a host-microbe interplay associated with insecticide resistance inFrontiers in microbiology · 2026Article
- Uridine diphosphate (UDP)-glycosyltransferases (UGTs) are associated with insecticide resistance in the major malaria vectors Anopheles gambiae s.l. and Anopheles funestus.Scientific reports · 2024Article
- Heat shock proteins, thermotolerance, and insecticide resistance in mosquitoes.Frontiers in insect science · 2024Review
- Insecticide resistance in the field populations of the Asian tiger mosquitoFrontiers in physiology · 2024Article
- RNA-Seq-Pop: Exploiting the sequence in RNA sequencing-A Snakemake workflow reveals patterns of insecticide resistance in the malaria vector Anopheles gambiae.Molecular ecology resources · 2023Article
- Identification of differentially expressed genes based on antennae RNA-seq analyses in Culex quinquefasciatus and Culex pipiens molestus.Parasites & vectors · 2022Article
- Response of xenobiotic biodegradation and metabolic genes in Tribolium castaneum following eugenol exposure.Molecular genetics and genomics : MGG · 2022Article
- The Differential Metabolic Profiles Between Deltamethrin-Resistant and -Susceptible Strains of Aedes albopictus (Diptera: Culicidae) by 1H-NMR.Journal of medical entomology · 2021Article
- From Global to Local-New Insights into Features of Pyrethroid Detoxification in Vector Mosquitoes.Insects · 2021Review
- Comparative proteomics reveals mechanisms that underlie insecticide resistance in Culex pipiens pallens Coquillett.PLoS neglected tropical diseases · 2021Article
- Molecular basis of resistance to organophosphate insecticides in the New World screw-worm fly.Parasites & vectors · 2020Article
- Transcription factor FTZ-F1 regulates mosquito cuticular protein CPLCG5 conferring resistance to pyrethroids in Culex pipiens pallens.Parasites & vectors · 2020Article
- Insecticidal Activity ofFrontiers in genetics · 2020Article
- Lipase is associated with deltamethrin resistance in Culex pipiens pallens.Parasitology research · 2020Article
- Identification and classification of differentially expressed genes in pyrethroid-resistant Culex pipiens pallens.Molecular genetics and genomics : MGG · 2019Article
- Comparative transcriptome analysis and RNA interference reveal CYP6A8 and SNPs related to pyrethroid resistance in Aedes albopictus.PLoS neglected tropical diseases · 2018Article
- The Effect of Permethrin Resistance onViruses · 2018Article
- De novo RNA-seq and functional annotation of Ornithonyssus bacoti.Experimental & applied acarology · 2018Article
- Culex pipiens pallens cuticular protein CPLCG5 participates in pyrethroid resistance by forming a rigid matrix.Parasites & vectors · 2018Article
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Corrections and comments
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Authors and funding
15 authors at 1 institution in 1 country.
Funding
Abstract
The widespread and improper use of pyrethroid insecticides, such as deltamethrin, has resulted in the evolution of resistance in many mosquito species, including Culex pipiens pallens. With the development of high-throughput sequencing, it is possible to massively screen pyrethroid resistance-associated gene. In this study, we used Illumina-Solexa transcriptome sequencing to identify genes that are expressed differently in deltamethrin-susceptible and -resistant strains of Culex pipiens pallens as a critical knowledge base for further studies. A total of 4,961,197,620 base pairs and 55,124,418 reads were sequenced, mapped to the Culex quinquefasciatus genome and assembled into 17,679 known genes. We recorded 1826 significantly differentially expressed genes (DEGs). Among them, 1078 genes were up-regulated and 748 genes were down-regulated in the deltamethrin-resistant strain compared to -susceptible strain. These DEGs contained cytochrome P450 s, cuticle proteins, UDP-glucuronosyltransferases, lipases, serine proteases, heat shock proteins, esterases and others. Among the 1826 DEGs, we found that the transcriptional levels of CYP6AA9 in the laboratory populations was elevated as the levels of deltamethrin resistance increased. Moreover, the expression levels of the CYP6AA9 were significantly higher in the resistant strains than the susceptible strains in three different field populations. We further confirmed the association between the CYP6AA9 gene and deltamethrin resistance in mosquitoes by RNA interfering (RNAi). Altogether, we explored massive potential pyrethroid resistance-associated genes and demonstrated that CYP6AA9 participated in the pyrethroid resistance in mosquitoes.
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