Evidence map›Paper›PMID 26377802›Full record

ArticleJournal of neuroinflammation2015

Inhibition of NOX2 reduces locomotor impairment, inflammation, and oxidative stress after spinal cord injury.

Guzal Khayrullina, Sara Bermudez, Kimberly R Byrnes

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of neuroinflammation, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
67citing papers in PubMed, 1 pooled it
6.0field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

67 citing papers in PubMed, 1 synthesis or guideline pooled it, 92 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Review
  4. Article
  5. Review
  6. Review
  7. Review
  8. Review
  9. Review
  10. Review
  11. Review
  12. Review
  13. Review
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Review

7 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 2 countries.

Guzal KhayrullinaAnatomy, Physiology and Genetics Department, Uniformed Services University, Room B2048, 4301 Jones Bridge Road, Bethesda, MD, 20814, USA. Guzal.khayrullina.ctr@usuhs.edu.
Sara BermudezAnatomy, Physiology and Genetics Department, Uniformed Services University, Room B2048, 4301 Jones Bridge Road, Bethesda, MD, 20814, USA. sara.bermudez.ctr@usuhs.edu.
Kimberly R ByrnesAnatomy, Physiology and Genetics Department, Uniformed Services University, Room B2048, 4301 Jones Bridge Road, Bethesda, MD, 20814, USA. kimberly.byrnes@usuhs.edu.
Uniformed Services University of the Health Sciences · USUnifor · NO

Funding

The NADPH oxidase enzyme as a therapeutic target after Spinal Cord InjuryR01NS073667 · NINDS · HENRY M. JACKSON FDN FOR THE ADV MIL/MED · PI BYRNES, KIMBERLY · 2012 to 2016
$1.3M
NINDS NIH HHS 1R01NS073667-01A1NINDS NIH HHS R01 NS073667
6 · The paper itself

Abstract

backgroundSpinal cord injury (SCI) results in the activation of the NADPH oxidase (NOX) enzyme, inducing production of reactive oxygen species (ROS). We hypothesized that the NOX2 isoform plays an integral role in post-SCI inflammation and functional deficits.

methodsModerate spinal cord contusion injury was performed in adult male mice, and flow cytometry, western blot, and immunohistochemistry were used to assess NOX2 activity and expression, inflammation, and M1/M2 microglia/macrophage polarization from 1 to 28 days after injury. The NOX2-specific inhibitor, gp91ds-tat, was injected into the intrathecal space immediately after impact. The Basso Mouse Scale (BMS) was used to assess locomotor function at 24 h post-injury and weekly thereafter.

resultsOur findings show that gp91ds-tat treatment significantly improved functional recovery through 28 days post-injury and reduced inflammatory cell concentrations in the injured spinal cord at 24 h and 7 days post-injury. In addition, a number of oxidative stress markers were reduced in expression at 24 h after gp91ds-tat treatment, which was accompanied by a reduction in M1 polarization marker expression.

conclusionBased on our findings, we now conclude that inhibition of NOX2 significantly improves outcome after SCI, most likely via acute reductions in oxidative stress and inflammation. NOX2 inhibition may therefore have true potential as a therapy after SCI.

Indexed as

Analysis of VarianceAnimalsAntigens, CDCalcium-Binding ProteinsDisease Models, AnimalFlow CytometryGlycoproteinsInflammationMacrophagesMaleMembrane GlycoproteinsMiceMice, Inbred C57BLMicrofilament ProteinsMicrogliaMovement DisordersAif1 protein, mouseAntigens, CDCalcium-Binding ProteinsCybb protein, mouseGlycoproteinsgp91ds-tat protein, chimericMembrane GlycoproteinsMicrofilament ProteinsNADPH Oxidase 2NADPH OxidasesReactive Oxygen Species

Identifiers

PMID26377802
PMCPMC4574142
OpenAlexW1889485241

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.