ArticleBMC genomics2015
Identification of candidate gonadal sex differentiation genes in the chicken embryo using RNA-seq.
Article in BMC genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 98 citations in OpenAlex.
- Mechanistic understanding of female reproductive aging based on the chicken model.Journal of animal science and biotechnology · 2026Review
- Evolution of a ZW sex determination system in sticklebacks.Science advances · 2026Article
- Article
- Single-nucleus transcriptional and chromatin accessible profiles reveal critical cell types and molecular architecture underlying chicken sex determination.Journal of advanced research · 2025Article
- Article
- The full-length transcriptional of the multiple spatiotemporal embryo-gonad tissues in chicken (Gallus gallus).BMC genomic data · 2024Article
- Comprehensive Annotation and Expression Profiling of C2H2 Zinc Finger Transcription Factors across Chicken Tissues.International journal of molecular sciences · 2024Article
- The Omics Revolution in Understanding Chicken Reproduction: A Comprehensive Review.Current issues in molecular biology · 2024Review
- Review
- RNA sequencing analysis of sexual dimorphism in Japanese quail.Frontiers in veterinary science · 2024Article
- Extraordinary variability in gene activation and repression programs during gonadal sex differentiation across vertebrates.Frontiers in cell and developmental biology · 2024Article
- Gene Expression Profiling Reveals Potential Players of Sex Determination and Asymmetrical Development in Chicken Embryo Gonads.International journal of molecular sciences · 2023Article
- DMRT1-mediated regulation of TOX3 modulates expansion of the gonadal steroidogenic cell lineage in the chicken embryo.Development (Cambridge, England) · 2023Article
- Transcriptomic analysis reveals the dynamic changes of transcription factors during early development of chicken embryo.BMC genomics · 2022Article
- Ancient homomorphy of molluscan sex chromosomes sustained by reversible sex-biased genes and sex determiner translocation.Nature ecology & evolution · 2022Article
- Improved biomarker discovery through a plot twist in transcriptomic data analysis.BMC biology · 2022Article
- Prediction of sex-determination mechanisms in avian primordial germ cells using RNA-seq analysis.Scientific reports · 2022Article
- H3K27ac chromatin acetylation and gene expression analysis reveal sex- and situs-related differences in developing chicken gonads.Biology of sex differences · 2022Article
- Fadrozole-mediated sex reversal in the embryonic chicken gonad involves a PAX2 positive undifferentiated supporting cell state.Frontiers in cell and developmental biology · 2022Article
- Time-Course Transcriptional and Chromatin Accessibility Profiling Reveals Genes Associated With Asymmetrical Gonadal Development in Chicken Embryos.Frontiers in cell and developmental biology · 2022Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDespite some advances in recent years, the genetic control of gonadal sex differentiation during embryogenesis is still not completely understood. To identify new candidate genes involved in ovary and testis development, RNA-seq was used to define the transcriptome of embryonic chicken gonads at the onset of sexual differentiation (day 6.0/stage 29).
resultsRNA-seq revealed more than 1000 genes that were transcribed in a sex-biased manner at this early stage of gonadal differentiation. Comparison with undifferentiated gonads revealed that sex biased expression was derived primarily from autosomal rather than sex-linked genes. Gene ontology and pathway analysis indicated that many of these genes encoded proteins involved in extracellular matrix function and cytoskeletal remodelling, as well as tubulogenesis. Several of these genes are novel candidate regulators of gonadal sex differentiation, based on sex-biased expression profiles that are altered following experimental sex reversal. We further characterised three female-biased (ovarian) genes; calpain-5 (CAPN5), G-protein coupled receptor 56 (GPR56), and FGFR3 (fibroblast growth factor receptor 3). Protein expression of these candidates in the developing ovaries suggests that they play an important role in this tissue.
conclusionsThis study provides insight into the earliest steps of vertebrate gonad sex differentiation, and identifies novel candidate genes for ovarian and testicular development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.