Evidence map›Paper›PMID 26377490›Full record

ArticleInternational urology and nephrology2015

Sildenafil activates antioxidant and antiapoptotic genes and inhibits proinflammatory cytokine genes in a rat model of renal ischemia/reperfusion injury.

Mohamed H Zahran, Abdelaziz M Hussein, Nashwa Barakat, Amira Awadalla, Shery Khater, Ahmed Harraz, Ahmed A Shokeir

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Article in International urology and nephrology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed, 1 pooled it
2.8field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 1 synthesis or guideline pooled it, 61 citations in OpenAlex.

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  15. Reno-protective effects of Phosphodiesterase 5 inhibitors.Clinical and experimental nephrology · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Mohamed H ZahranUrology and Nephrology Center, Mansoura University, Mansoura, Egypt.
Abdelaziz M HusseinPhysiology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt. zizomenna28@yahoo.com.
Nashwa BarakatUrology and Nephrology Center, Mansoura University, Mansoura, Egypt.
Amira AwadallaUrology and Nephrology Center, Mansoura University, Mansoura, Egypt.
Shery KhaterUrology and Nephrology Center, Mansoura University, Mansoura, Egypt.
Ahmed HarrazUrology and Nephrology Center, Mansoura University, Mansoura, Egypt.
Ahmed A ShokeirUrology and Nephrology Center, Mansoura University, Mansoura, Egypt.
Mansoura University · EG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesTo study the possible renoprotective effect of sildenafil against renal ischemia/reperfusion (I/R) injury and its effect on the expression of some antioxidant, antiapoptotic gene and proinflammatory cytokine genes in rat model of renal I/R injury. MATERIALS AND

methodsOne hundred and twenty male Sprague Dawley rats were subdivided into three equal groups: sham (underwent right nephrectomy without ischemia), control (underwent right nephrectomy and left ischemia for 45 min) and study [as control with 1 mg/kg sildenafil (per oral) 60 min before anesthesia]. Serum creatinine and BUN were measured at the baseline and the study endpoints (2, 24, 48 h and 7 days), and the left kidney was harvested at study endpoints for histopathological examination as well as for assessment of the expression of antioxidant genes (Nrf-2, HO-1 and NQO-1), antiapoptotic gene (Bcl-2) and inflammatory cytokines, e.g., TNF-a, IL-1β and ICAM-1.

resultsI/R caused significant increase in serum creatinine, BUN, histopathological damage score (p < 0.001) and significant reduction in antioxidant genes (nrf2, HO-1 and NQO-1) and antiapoptotic gene (Bcl2) with significant increase in TNF-a, IL-1β and ICAM-1 genes in kidney tissues. Pretreatment with sildenafil caused significant attenuation of serum creatinine and BUN as well as significant increase in the expression of antioxidant genes and Bcl-2 genes with significant reduction in the expression of proinflammatory cytokine genes (p value < 0.001).

conclusionThe renoprotective effect of sildenafil against renal I/R might be due to the activation of antioxidant genes (Nrf2, HO-1 and NQO-1) and antiapoptotic gene (Bcl2) and attenuation of proinflammatory cytokines (TNF-a, IL-1β and ICAM-1).

Indexed as

AnimalsBlood Urea NitrogenCreatinineDisease Models, AnimalGene ExpressionHeme Oxygenase-1Intercellular Adhesion Molecule-1Interleukin-1betaMaleNAD(P)H Dehydrogenase (Quinone)NephrectomyNF-E2-Related Factor 2Phosphodiesterase 5 InhibitorsProto-Oncogene Proteins c-bcl-2RatsRats, Sprague-DawleyCreatinineHeme Oxygenase-1Intercellular Adhesion Molecule-1Interleukin-1betaNAD(P)H Dehydrogenase (Quinone)Nfe2l2 protein, ratNF-E2-Related Factor 2NQO1 protein, ratPhosphodiesterase 5 InhibitorsProto-Oncogene Proteins c-bcl-2Sildenafil CitrateTumor Necrosis Factor-alphaBcl-2HO-1Ischemia/reperfusionKidneyNQO-1Nrf2Sildenafil

Identifiers

PMID26377490
OpenAlexW1720082734

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.