ArticleBiology of reproduction2015
Cigarette smoke exposure triggers the autophagic cascade via activation of the AMPK pathway in mice.
Article in Biology of reproduction, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 53 citations in OpenAlex.
- Waterpipe smoke condensate activates autophagy in non-tumorigenic lung epithelial cells: Implications for inflammation and stemness.PloS one · 2026Article
- Dietary, metabolic and gut microbiota influences on primary ovarian failure: a two-sample Mendelian randomization study.Asia Pacific journal of clinical nutrition · 2025Article
- AMPK, a key molecule regulating aging-related myocardial ischemia-reperfusion injury.Molecular biology reports · 2024Review
- MiR-17-5p/FOXL2/CDKN1B signal programming in oocytes mediates transgenerational inheritance of diminished ovarian reserve in female offspring rats induced by prenatal dexamethasone exposure.Cell biology and toxicology · 2023Article
- Effects of N-acetylcysteine on Growth, Viability, and Ultrastructure of In Vitro Cultured Bovine Secondary Follicles.Animals : an open access journal from MDPI · 2022Article
- Aquaporin-8 transports hydrogen peroxide to regulate granulosa cell autophagy.Frontiers in cell and developmental biology · 2022Article
- Metformin: Experimental and Clinical Evidence for a Potential Role in Emphysema Treatment.American journal of respiratory and critical care medicine · 2021Observational
- In vitro study of the role of FOXO transcription factors in regulating cigarette smoke extract-induced autophagy.Cell biology and toxicology · 2021Article
- Protective Mechanism of Luteinizing Hormone and Follicle-Stimulating Hormone Against Nicotine-Induced Damage of Mouse Early Folliculogenesis.Frontiers in cell and developmental biology · 2021Article
- Correlation between level of autophagy and frequency of CD8The Journal of international medical research · 2020Article
- Looking Inside the World of Granulosa Cells: The Noxious Effects of Cigarette Smoke.Biomedicines · 2020Review
- Ovarian mitochondrial dynamics and cell fate regulation in an androgen-induced rat model of polycystic ovarian syndrome.Scientific reports · 2020Article
- Article
- Proposed Key Characteristics of Female Reproductive Toxicants as an Approach for Organizing and Evaluating Mechanistic Data in Hazard Assessment.Environmental health perspectives · 2019Article
- Exposure to polycyclic aromatic hydrocarbons derived from vehicle exhaust gas induces premature senescence in mouse lung fibroblast cells.Molecular medicine reports · 2019Article
- qPhos: a database of protein phosphorylation dynamics in humans.Nucleic acids research · 2019Article
- Identification of microRNAs as potential markers of ovarian toxicity.Journal of applied toxicology : JAT · 2018Article
- Protective effects of melatonin against nicotine-induced disorder of mouse early folliculogenesis.Aging · 2018Article
- The Orphan Nuclear Receptor Liver Homolog Receptor-1 (Nr5a2) Regulates Ovarian Granulosa Cell Proliferation.Journal of the Endocrine Society · 2018Article
- Cigarette smoke promotes COPD by activating platelet-activating factor receptor and inducing neutrophil autophagic death in mice.Oncotarget · 2017Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We previously demonstrated that cigarette smoke (CS) exposure decreases primordial follicle counts and induces autophagy in ovarian granulosa cells in preference to apoptosis. Therefore, the objective of this study was to investigate molecular targets underlying smoke-induced activation of the reparative autophagy pathway in the ovary. Briefly, ovarian homogenates were prepared from adult female mice exposed to mainstream CS twice daily for 8 wk, using a whole-body exposure system. A gene array revealed that CS exposure induced a greater than 2-fold significant increase in the expression of proautophagic genes Cdkn1b, Map1lc3a, Bad, and Sqstm1/p62. A significant increase in Prkaa2, Pik3c3, and Maplc31b expression, as well as a significant decrease in Akt1 and Mtor expression, was detected by quantitative PCR. The 5'-AMP-activated protein kinase catalytic subunit (AMPK) alpha1 + alpha2 and ATG7 protein expression was significantly increased, whereas AKT1, mTOR, CDKN1B/p27, and CXCR4 proteins were significantly decreased in CS exposed versus control ovaries. Up-regulation of AMPK alpha1 + alpha2, a known initiator of autophagic signaling, and ATG7 further suggests activation of the autophagy cascade. Two prosurvival factors, AKT and mTOR, were decreased in expression, an outcome that favors induction of the autophagy pathway, whereas decreased levels of CDKN1B is suggestive of cell cycle dysregulation. In summary, our data suggest that CS exposure induces ovarian follicle loss through induction of the autophagic cascade via the AMPK pathway together with inhibition of antiautophagic markers AKT and mTOR. We further postulate that toxicant-induced dysregulation of reparative autophagy is a novel pathway central to impaired follicle development and subfertility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.