ArticleAngewandte Chemie (International ed. in English)2015
Enzymology of Pyran Ring A Formation in Salinomycin Biosynthesis.
Article in Angewandte Chemie (International ed. in English), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Discovery of a lagriamide polyketide by integrated genome mining, isotopic labeling, and untargeted metabolomics.Chemical science · 2024Article
- Modifications of Protein-Bound Substrates by Trans-Acting Enzymes in Natural Products Biosynthesis.Chembiochem : a European journal of chemical biology · 2024Review
- Article
- Reconstitution of a mini-gene cluster combined with ribosome engineering led to effective enhancement of salinomycin production in Streptomyces albus.Microbial biotechnology · 2021Article
- Enzymatic Cascade Reactions in Biosynthesis.Angewandte Chemie (International ed. in English) · 2019Review
- An antifungal polyketide associated with horizontally acquired genes supports symbiont-mediated defense in Lagria villosa beetles.Nature communications · 2018Article
- Recent Advances in Enzymatic Complexity Generation: Cyclization Reactions.Biochemistry · 2018Review
- Structural and Functional Studies of a Pyran Synthase Domain from a trans-Acyltransferase Assembly Line.ACS chemical biology · 2018Article
- The Uncommon Enzymology of Cis-Acyltransferase Assembly Lines.Chemical reviews · 2017Review
- The Draft Genome Sequence of Actinokineospora bangkokensis 44EHWMolecules (Basel, Switzerland) · 2016Article
- Stereocontrol within polyketide assembly lines.Natural product reports · 2016Review
- Zincophorin - biosynthesis in Streptomyces griseus and antibiotic properties.GMS infectious diseases · 2016Article
- Evidence for an iterative module in chain elongation on the azalomycin polyketide synthase.Beilstein journal of organic chemistry · 2016Article
- Biosynthesis of oxygen and nitrogen-containing heterocycles in polyketides.Beilstein journal of organic chemistry · 2016Review
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Authors and funding
6 authors.
Funding
Abstract
Tetrahydropyran rings are a common feature of complex polyketide natural products, but much remains to be learned about the enzymology of their formation. The enzyme SalBIII from the salinomycin biosynthetic pathway resembles other polyether epoxide hydrolases/cyclases of the MonB family, but SalBIII plays no role in the conventional cascade of ring opening/closing. Mutation in the salBIII gene gave a metabolite in which ring A is not formed. Using this metabolite in vitro as a substrate analogue, SalBIII has been shown to form pyran ring A. We have determined the X-ray crystal structure of SalBIII, and structure-guided mutagenesis of putative active-site residues has identified Asp38 and Asp104 as an essential catalytic dyad. The demonstrated pyran synthase activity of SalBIII further extends the impressive catalytic versatility of α+β barrel fold proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.