ArticleMicrobial cell factories2015
A Streptomyces coelicolor host for the heterologous expression of Type III polyketide synthase genes.
Article in Microbial cell factories, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 45 citations in OpenAlex.
- Review
- Comparative genomic and phenotypic analysis of Streptomyces rochei SOSIST-3 isolated from the Southern Ocean.Molecular biology reports · 2026Article
- Application of multiple genomic-editing technologies inSynthetic and systems biotechnology · 2025Article
- Synthetic Biology in Natural Product Biosynthesis.Chemical reviews · 2025Review
- Consequences of the deletion of the major specialized metabolite biosynthetic pathways of Streptomyces coelicolor on the metabolome and lipidome of this strain.Microbial biotechnology · 2024Article
- Respiratory Quinone Switches from Menaquinone to Polyketide Quinone during the Development Cycle inMicrobiology spectrum · 2023Article
- Complete Genome Analysis of Undecylprodigiosin Pigment Biosynthesizing MarineMicroorganisms · 2021Article
- Review
- A chromatogram-simplified Streptomyces albus host for heterologous production of natural products.Antonie van Leeuwenhoek · 2020Article
- Engineering Biology to Construct Microbial Chassis for the Production of Difficult-to-Express Proteins.International journal of molecular sciences · 2020Review
- Novel bioactive natural products from bacteria via bioprospecting, genome mining and metabolic engineering.Microbial biotechnology · 2019Review
- Antarctic Streptomyces fildesensis So13.3 strain as a promising source for antimicrobials discovery.Scientific reports · 2019Article
- Recent Advances in Synthetic Biology Approaches to Optimize Production of Bioactive Natural Products in Actinobacteria.Frontiers in microbiology · 2019Review
- Output ordering and prioritisation system (OOPS): ranking biosynthetic gene clusters to enhance bioactive metabolite discovery.Journal of industrial microbiology & biotechnology · 2018Article
- Discovery of Unusual Biaryl Polyketides by Activation of a Silent Streptomyces venezuelae Biosynthetic Gene Cluster.Chembiochem : a European journal of chemical biology · 2016Article
- Identification of a novel strain, Streptomyces blastmyceticus JZB130180, and evaluation of its biocontrol efficacy against Monilinia fructicola.Journal of Zhejiang University. Science. BArticle
- Streptomycetes: Surrogate hosts for the genetic manipulation of biosynthetic gene clusters and production of natural products.Biotechnology advancesReview
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5 authors at 3 institutions in 3 countries.
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Abstract
backgroundRecent advances in genome sequencing, combined with bioinformatic analysis, has led to the identification of numerous novel natural product gene clusters, particularly in actinomycetes of terrestrial and marine origin. Many of these gene clusters encode uncharacterised Type III polyketide synthases. To facilitate the study of these genes and their potentially novel products, we set out to construct an actinomycete expression host specifically designed for the heterologous expression of Type III PKS genes and their gene clusters.
resultsA derivative of Streptomyces coelicolor A3(2) designed for the expression of Type III polyketide synthase (PKS) genes was constructed from the previously engineered expression strain S. coelicolor M1152 [Δact Δred Δcpk Δcda rpoB(C1298T)] by removal of all three of the endogenous Type III PKS genes (gcs, srsA, rppA) by PCR targeting. The resulting septuple deletion mutant, M1317, proved to be an effective surrogate host for the expression of actinobacterial Type III PKS genes: expression of the reintroduced gcs gene from S. coelicolor and of the heterologous rppA gene from Streptomyces venezuelae under the control of the constitutive ermE* promoter resulted in copious production of germicidin and flaviolin, respectively.
conclusionsThe newly constructed expression host S. coelicolor M1317 should be particularly useful for the discovery and analysis of new Type III polyketide metabolites.
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