ArticleMucosal immunology2016
Assessing DNA methylation in the developing human intestinal epithelium: potential link to inflammatory bowel disease.
Article in Mucosal immunology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
44 citing papers in PubMed, 91 citations in OpenAlex.
- Mucosal DNA methylation reveals immune-related methylation profile and correlates with crohn's disease status.Scientific reports · 2026Article
- Epigenetic modulation of the NLRP6 inflammasome sensor as a therapeutic modality to reduce necroptosis-driven gastrointestinal mucosal dysfunction in HIV/SIV infection.Cell communication and signaling : CCS · 2025Article
- The Role of DNA Methylation in Gastrointestinal Disease: An Expanded Review of Malignant and Nonmalignant Gastrointestinal Diseases.Gastroenterology · 2025Review
- Mitochondrial function and gastrointestinal diseases.Nature reviews. Gastroenterology & hepatology · 2024Review
- α-Ketoglutarate for Preventing and Managing Intestinal Epithelial Dysfunction.Advances in nutrition (Bethesda, Md.) · 2024Review
- INVITED COMMENTARY on Andersen S, et al. Developmental Windows of Environmental Vulnerability for Inflammatory Bowel Disease.Journal of pediatrics. Clinical practice · 2024Article
- Article
- Analysis of Systemic Epigenetic Alterations in Inflammatory Bowel Disease: Defining Geographical, Genetic and Immune-Inflammatory influences on the Circulating Methylome.Journal of Crohn's & colitis · 2023Article
- Article
- Mucosal DNA methylome alteration in Crohn's disease: surgical and non-surgical groups.Frontiers in genetics · 2023Article
- Review
- DNA methyltransferase 3A controls intestinal epithelial barrier function and regeneration in the colon.Nature communications · 2022Article
- Epigenetic DNA methylation of Zbtb7b regulates the population of double-positive CD4Journal of translational medicine · 2022Article
- Challenges in Analyzing Functional Epigenetic Data in Perspective of Adolescent Psychiatric Health.International journal of molecular sciences · 2022Review
- Genome-wide DNA methylation profiling in differentiating Crohn's disease from intestinal tuberculosis.Genes & genomics · 2022Article
- The Role of Oxidative Stress in Epigenetic Changes Underlying Autoimmunity.Antioxidants & redox signaling · 2022Review
- A Black Raspberry-Rich Diet Protects From Dextran Sulfate Sodium-Induced Intestinal Inflammation and Host Metabolic Perturbation in Association With Increased Aryl Hydrocarbon Receptor Ligands in the Gut Microbiota of Mice.Frontiers in nutrition · 2022Article
- Epigenetics in IBD: a conceptual framework for disease pathogenesis.Frontline gastroenterology · 2022Article
- Culture-Associated DNA Methylation Changes Impact on Cellular Function of Human Intestinal Organoids.Cellular and molecular gastroenterology and hepatology · 2022Article
- New Insights Into the Epigenetic Regulation of Inflammatory Bowel Disease.Frontiers in pharmacology · 2022Review
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA methylation is one of the major epigenetic mechanisms implicated in regulating cellular development and cell-type-specific gene expression. Here we performed simultaneous genome-wide DNA methylation and gene expression analysis on purified intestinal epithelial cells derived from human fetal gut, healthy pediatric biopsies, and children newly diagnosed with inflammatory bowel disease (IBD). Results were validated using pyrosequencing, real-time PCR, and immunostaining. The functional impact of DNA methylation changes on gene expression was assessed by employing in-vitro assays in intestinal cell lines. DNA methylation analyses allowed identification of 214 genes for which expression is regulated via DNA methylation, i.e. regulatory differentially methylated regions (rDMRs). Pathway and functional analysis of rDMRs suggested a critical role for DNA methylation in regulating gene expression and functional development of the human intestinal epithelium. Moreover, analysis performed on intestinal epithelium of children newly diagnosed with IBD revealed alterations in DNA methylation within genomic loci, which were found to overlap significantly with those undergoing methylation changes during intestinal development. Our study provides novel insights into the physiological role of DNA methylation in regulating functional maturation of the human intestinal epithelium. Moreover, we provide data linking developmentally acquired alterations in the DNA methylation profile to changes seen in pediatric IBD.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.