ArticlePloS one2015
Biochemical Characterization of Putative Adenylate Dimethylallyltransferase and Cytokinin Dehydrogenase from Nostoc sp. PCC 7120.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- How Do Plant Growth-Promoting Bacteria Use Plant Hormones to Regulate Stress Reactions?Plants (Basel, Switzerland) · 2024Review
- Zeatin: The 60th anniversary of its identification.Plant physiology · 2023Article
- Article
- The origin and early evolution of cytokinin signaling.Frontiers in plant science · 2023Review
- Cytokinin biosynthesis in cyanobacteria: Insights for crop improvement.Frontiers in genetics · 2022Review
- Biochemical and Structural Aspects of Cytokinin Biosynthesis and Degradation in Bacteria.Microorganisms · 2021Review
- The Hulks and the Deadpools of the Cytokinin Universe: A Dual Strategy for Cytokinin Production, Translocation, and Signal Transduction.Biomolecules · 2021Review
- Review
- Cytokinin oxidase PpCKX1 plays regulatory roles in development and enhances dehydration and salt tolerance in Physcomitrella patens.Plant cell reports · 2020Article
- Cytokinins in Dictyostelia - A Unique Model for Studying the Functions of Signaling Agents From Species to Kingdoms.Frontiers in cell and developmental biology · 2020Review
- Occurrence and biosynthesis of cytokinins in poplar.Planta · 2019Article
- Tangled history of a multigene family: The evolution of ISOPENTENYLTRANSFERASE genes.PloS one · 2018Article
- Control of cytokinin and auxin homeostasis in cyanobacteria and algae.Annals of botany · 2017Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytokinins, a class of phytohormones, are adenine derivatives common to many different organisms. In plants, these play a crucial role as regulators of plant development and the reaction to abiotic and biotic stress. Key enzymes in the cytokinin synthesis and degradation in modern land plants are the isopentyl transferases and the cytokinin dehydrogenases, respectively. Their encoding genes have been probably introduced into the plant lineage during the primary endosymbiosis. To shed light on the evolution of these proteins, the genes homologous to plant adenylate isopentenyl transferase and cytokinin dehydrogenase were amplified from the genomic DNA of cyanobacterium Nostoc sp. PCC 7120 and expressed in Escherichia coli. The putative isopentenyl transferase was shown to be functional in a biochemical assay. In contrast, no enzymatic activity was detected for the putative cytokinin dehydrogenase, even though the principal domains necessary for its function are present. Several mutant variants, in which conserved amino acids in land plant cytokinin dehydrogenases had been restored, were inactive. A combination of experimental data with phylogenetic analysis indicates that adenylate-type isopentenyl transferases might have evolved several times independently. While the Nostoc genome contains a gene coding for protein with characteristics of cytokinin dehydrogenase, the organism is not able to break down cytokinins in the way shown for land plants.
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