ArticleIntegrative biology : quantitative biosciences from nano to macro2015
Band-pass processing in a GPCR signaling pathway selects for NFAT transcription factor activation.
Article in Integrative biology : quantitative biosciences from nano to macro, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Who cites it
9 citing papers in PubMed, 19 citations in OpenAlex.
- Pulsatile FXR activation: Timing bile acid-targeted therapy to physiology.Acta pharmacologica Sinica · 2026Article
- Frequency-preference response in covalent modification cycles under substrate sequestration conditions.NPJ systems biology and applications · 2021Article
- Selective Photoswitchable Allosteric Agonist of a G Protein-Coupled Receptor.Journal of the American Chemical Society · 2021Article
- Genetically Targeted Optical Control of an Endogenous G Protein-Coupled Receptor.Journal of the American Chemical Society · 2019Article
- Camphor white oil induces tumor regression through cytotoxic T cell-dependent mechanisms.Molecular carcinogenesis · 2019Article
- A Two-Pulse Cellular Stimulation Test Elucidates Variability and Mechanisms in Signaling Pathways.Biophysical journal · 2019Article
- Article
- Computing Mathematical Functions using DNA via Fractional Coding.Scientific reports · 2018Article
- New insights into mammalian signaling pathways using microfluidic pulsatile inputs and mathematical modeling.Integrative biology : quantitative biosciences from nano to macro · 2017Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Many biological processes are rhythmic and proper timing is increasingly appreciated as being critical for development and maintenance of physiological functions. To understand how temporal modulation of an input signal influences downstream responses, we employ microfluidic pulsatile stimulation of a G-protein coupled receptor, the muscarinic M3 receptor, in single cells with simultaneous real-time imaging of both intracellular calcium and NFAT nuclear localization. Interestingly, we find that reduced stimulation with pulses of ligand can give more efficient transcription factor activation, if stimuli are timed appropriately. Our experiments and computational analyses show that M3 receptor-induced calcium oscillations form a low pass filter while calcium-induced NFAT translocation forms a high pass filter. The combination acts as a band-pass filter optimized for intermediate frequencies of stimulation. We demonstrate that receptor desensitization and NFAT translocation rates determine critical features of the band-pass filter and that the band-pass may be shifted for different receptors or NFAT dynamics. As an example, we show that the two NFAT isoforms (NFAT4 and NFAT1) have shifted band-pass windows for the same receptor. While we focus specifically on the M3 muscarinic receptor and NFAT translocation, band-pass processing is expected to be a general theme that applies to multiple signaling pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.