ArticleScientific reports2015
Background-free in-vivo Imaging of Vitamin C using Time-gateable Responsive Probe.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 43 citations in OpenAlex.
- Vitamin-based radiopharmaceuticals for tumor imaging.Medical oncology (Northwood, London, England) · 2023Review
- Quantitative tracing of uptake and transport of submicrometre plastics in crop plants using lanthanide chelates as a dual-functional tracer.Nature nanotechnology · 2022Article
- Review
- Opportunities for Persistent Luminescent Nanoparticles in Luminescence Imaging of Biological Systems and Photodynamic Therapy.Nanomaterials (Basel, Switzerland) · 2020Review
- Effect of the Heteroaromatic Antenna on the Binding of Chiral Eu(III) Complexes to Bovine Serum Albumin.Inorganic chemistry · 2020Article
- Lanthanide-Based Optical Probes of Biological Systems.Cell chemical biology · 2020Review
- Activity-Based Sensing of Ascorbate by Using Copper-Mediated Oxidative Bond Cleavage.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020Article
- Recent Advances in Luminescence Imaging of Biological Systems Using Lanthanide(III) Luminescent Complexes.Molecules (Basel, Switzerland) · 2020Review
- Cobalt oxyhydroxide modified with poly-β-cyclodextrin and a cyanine dye as a nanoplatform for two-photon imaging of ascorbic acid in living cells and tissue.Mikrochimica acta · 2019Article
- In vivo fluorescence bioimaging of ascorbic acid in mice: Development of an efficient probe consisting of phthalocyanine, TEMPO, and albumin.Scientific reports · 2018Article
- Dual-emissive nanoarchitecture of lanthanide-complex-modified silica particles forChemical science · 2017Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sensitive optical imaging of active biomolecules in the living organism requires both a molecular probe specifically responsive to the target and a high-contrast approach to remove the background interference from autofluorescence and light scatterings. Here, a responsive probe for ascorbic acid (vitamin C) has been developed by conjugating two nitroxide radicals with a long-lived luminescent europium complex. The nitroxide radical withholds the probe on its "off" state (barely luminescent), until the presence of vitamin C will switch on the probe by forming its hydroxylamine derivative. The probe showed a linear response to vitamin C concentration with a detection limit of 9.1 nM, two orders of magnitude lower than that achieved using electrochemical methods. Time-gated luminescence microscopy (TGLM) method has further enabled real-time, specific and background-free monitoring of cellular uptake or endogenous production of vitamin C, and mapping of vitamin C in living Daphnia magna. This work suggests a rational design of lanthanide complexes for background-free small animal imaging of biologically functional molecules.
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