ArticlePlant cell reports2015
Transcriptomic profiling revealed the regulatory mechanism of Arabidopsis seedlings response to oxidative stress from cryopreservation.
Article in Plant cell reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Integrated transcriptomics and metabolomics elucidate the molecular mechanism underlying post-preservation pollen viability alterations in Paeonia lactiflora.BMC plant biology · 2025Article
- Light spectrum modulates stress and defense gene expression in potato (Solanum tuberosum L.) shoot tips during post-cryopreservation recovery.Scientific reports · 2025Article
- Molecular Mechanisms Underlying Freezing Tolerance in Plants: Implications for Cryopreservation.International journal of molecular sciences · 2024Review
- Article
- Characterization and transformation of TtMYB1 transcription factor from Tritipyrum to improve salt tolerance in wheat.BMC genomics · 2024Article
- Article
- Critical Role of Regrowth Conditions in Post-Cryopreservation of In Vitro Plant Germplasm.Biology · 2023Review
- Transcriptome Profiling during Sequential Stages of Cryopreservation in Banana (Plants (Basel, Switzerland) · 2023Article
- Enzymatic Antioxidant System Activation Assures the Viability ofPlants (Basel, Switzerland) · 2023Article
- Abscisic acid induces the expression of AsKIN during the recovery period of garlic cryopreservation.Plant cell reports · 2022Article
- Impacts of Rapid Desiccation on Oxidative Status, Ultrastructure and Physiological Functions ofPlants (Basel, Switzerland) · 2022Article
- Review
- The transcription factor WRKY22 is required during cryo-stress acclimation in Arabidopsis shoot tips.Journal of experimental botany · 2020Article
- The ROS-associated programmed cell death causes the decline of pollen viability recovered from cryopreservation in Paeonia lactiflora.Plant cell reports · 2020Article
- Single-wall carbon nanotubes improve cell survival rate and reduce oxidative injury in cryopreservation ofPlant methods · 2020Article
- Monitoring of oxidative status in three native Australian species during cold acclimation and cryopreservation.Plant cell reports · 2017Article
- Changes in transcript expression patterns as a result of cryoprotectant treatment and liquid nitrogen exposure in Arabidopsis shoot tips.Plant cell reports · 2017Article
- D27E mutation of VTC1 impairs the interaction with CSN5B and enhances ascorbic acid biosynthesis and seedling growth in Arabidopsis.Plant molecular biology · 2016Article
- Article
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
key messageElevated antioxidant status and positive abiotic stress response in dehydration enhance cell resistance to cryoinjury, and controlling oxidative damage via reactive oxygen species homeostasis maintenance leads to high survival. Cryoprotectants are important for cell survival in cryopreservation, but high concentrations can also cause oxidative stress. Adding vitamin C to the cryoprotectant doubled the survival ratio in Arabidopsis thaliana 60-h seedlings (seedlings after 60-h germination) cryopreservation. In this study, the metabolites and transcriptional profiling of 60-h seedlings were analyzed in both the control cryopreservation procedure (CCP) and an improved cryopreservation procedure (ICP) to reveal the mechanism of plant cell response to oxidative stress from cryopreservation. Reactive oxygen species (ROS) and peroxidation levels reached a peak after rapid cooling-warming in CCP, which were higher than that in ICP. In addition, gene regulation was significantly increased in CCP and decreased in ICP during rapid cooling-warming. Before cryogenic treatment, the number of specifically regulated genes was nearly 10 times higher in ICP dehydration than CCP dehydration. Among these genes, DREBs/CBFs were beneficial to cope with cryoinjury, and calcium-binding protein, OXI1, WRKY and MYB family members as key factors in ROS signal transduction activated the ROS-producing and ROS-scavenging networks including AsA-GSH and GPX cycles involved in scavenging H2O2. Finally, elevated antioxidant status and oxidative stress response in the improved dehydration enhanced seedling resistance to cryogenic treatment, maintained ROS homeostasis and improved cell recovery after cryopreservation.
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