ArticlePlant cell reports2016
CaMV-35S promoter sequence-specific DNA methylation in lettuce.
Article in Plant cell reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
- Endogenous constitutive promoters with a broad range of transcriptional activities for plant synthetic biology.Plant cell reports · 2026Article
- A Rapid Hairy Root-Based Platform for CRISPR/Cas Optimization and Guide RNA Validation in Lettuce.Plants (Basel, Switzerland) · 2026Article
- Monitoring the stability of transgene expression in lettuce using the RUBY reporter.Plant cell reports · 2025Article
- Enhanced folate content in bioengineered lettuce stably expressed under field conditions.Transgenic research · 2025Article
- An Improved Method forPlants (Basel, Switzerland) · 2025Article
- Advances in promoter engineering strategies for enhanced recombinant protein expression in plants.Frontiers in plant science · 2025Review
- Enterohemorrhagic Escherichia coli O157:H7 antigens produced in transgenic lettuce effective as an oral vaccine in mice.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- MAP30 transgenic tobacco lines: from silencing to inducing.Molecular biology reports · 2021Article
- Rational design and testing of abiotic stress-inducible synthetic promoters from poplar cis-regulatory elements.Plant biotechnology journal · 2021Article
- Lettuce-based production of an oral vaccine against porcine edema disease for the seed lot system.Plant biotechnology (Tokyo, Japan) · 2021Article
- Peculiarities of the Transformation ofFrontiers in plant science · 2021Review
- Impact of differential DNA methylation on transgene expression in cotton (Gossypium hirsutum L.) events generated by targeted sequence insertion.Plant biotechnology journal · 2019Article
- Impacts of elevated COScientific reports · 2017Article
- Generation of marker-free transgenic hexaploid wheat via an Agrobacterium-mediated co-transformation strategy in commercial Chinese wheat varieties.Plant biotechnology journal · 2017Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageWe found 35S promoter sequence-specific DNA methylation in lettuce. Additionally, transgenic lettuce plants having a modified 35S promoter lost methylation, suggesting the modified sequence is subjected to the methylation machinery. We previously reported that cauliflower mosaic virus 35S promoter-specific DNA methylation in transgenic gentian (Gentiana triflora × G. scabra) plants occurs irrespective of the copy number and the genomic location of T-DNA, and causes strong gene silencing. To confirm whether 35S-specific methylation can occur in other plant species, transgenic lettuce (Lactuca sativa L.) plants with a single copy of the 35S promoter-driven sGFP gene were produced and analyzed. Among 10 lines of transgenic plants, 3, 4, and 3 lines showed strong, weak, and no expression of sGFP mRNA, respectively. Bisulfite genomic sequencing of the 35S promoter region showed hypermethylation at CpG and CpWpG (where W is A or T) sites in 9 of 10 lines. Gentian-type de novo methylation pattern, consisting of methylated cytosines at CpHpH (where H is A, C, or T) sites, was also observed in the transgenic lettuce lines, suggesting that lettuce and gentian share similar methylation machinery. Four of five transgenic lettuce lines having a single copy of a modified 35S promoter, which was modified in the proposed core target of de novo methylation in gentian, exhibited 35S hypomethylation, indicating that the modified sequence may be the target of the 35S-specific methylation machinery.
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