Evidence map›Paper›PMID 26373535›Full record

ArticleBMC cancer2015

Coronin 2A (CRN5) expression is associated with colorectal adenoma-adenocarcinoma sequence and oncogenic signalling.

Raphael H Rastetter, Margit Blömacher, Uta Drebber, Marija Marko, Juliane Behrens, Roxana Solga, Sarah Hojeili, Kurchi Bhattacharya, Claudia M Wunderlich, F Thomas Wunderlich and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC cancer, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
0.8field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 15 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Article
  5. Article
  6. Molecular Pathogenesis of the Coronin Family:International journal of molecular sciences · 2021
    Article
  7. PRMT5 promotes colorectal cancer growth by interaction with MCM7.Journal of cellular and molecular medicine · 2021
    Article
  8. Article
  9. Article
  10. Review
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 3 institutions in 2 countries.

Raphael H RastetterCenter for Biochemistry, Institute of Biochemistry I, Medical Faculty, University of Cologne, Joseph-Stelzmann-Street 52, 50931, Cologne, Germany.
Margit BlömacherCenter for Biochemistry, Institute of Biochemistry I, Medical Faculty, University of Cologne, Joseph-Stelzmann-Street 52, 50931, Cologne, Germany.
Uta DrebberInstitute of Pathology, University Hospital of Cologne, 50931, Cologne, Germany.
Marija MarkoCenter for Biochemistry, Institute of Biochemistry I, Medical Faculty, University of Cologne, Joseph-Stelzmann-Street 52, 50931, Cologne, Germany.
Juliane BehrensCenter for Biochemistry, Institute of Biochemistry I, Medical Faculty, University of Cologne, Joseph-Stelzmann-Street 52, 50931, Cologne, Germany.
Roxana SolgaCenter for Biochemistry, Institute of Biochemistry I, Medical Faculty, University of Cologne, Joseph-Stelzmann-Street 52, 50931, Cologne, Germany.
Sarah HojeiliCenter for Biochemistry, Institute of Biochemistry I, Medical Faculty, University of Cologne, Joseph-Stelzmann-Street 52, 50931, Cologne, Germany.
Kurchi BhattacharyaCenter for Biochemistry, Institute of Biochemistry I, Medical Faculty, University of Cologne, Joseph-Stelzmann-Street 52, 50931, Cologne, Germany.
Claudia M WunderlichInstitute for Genetics, University of Cologne, 50674, Cologne, Germany.
F Thomas WunderlichMax Planck Institute for Neurological Research, 50931, Cologne, Germany.
Margarete OdenthalInstitute of Pathology, University Hospital of Cologne, 50931, Cologne, Germany.
Anja ZiemannCenter for Biochemistry, Institute of Biochemistry I, Medical Faculty, University of Cologne, Joseph-Stelzmann-Street 52, 50931, Cologne, Germany.
Ludwig EichingerCenter for Biochemistry, Institute of Biochemistry I, Medical Faculty, University of Cologne, Joseph-Stelzmann-Street 52, 50931, Cologne, Germany.
Christoph S ClemenCenter for Biochemistry, Institute of Biochemistry I, Medical Faculty, University of Cologne, Joseph-Stelzmann-Street 52, 50931, Cologne, Germany. christoph.clemen@uni-koeln.de.
University of Cologne · DEUniversity Hospital Cologne · DEMax Planck Institute for Metabolism Research · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCoronin proteins are known as regulators of actin-based cellular processes, and some of them are associated with the malignant progression of human cancer. Here, we show that expression of coronin 2A is up-regulated in human colon carcinoma.

methodsThis study included 26 human colon tumour specimens and 9 normal controls. Expression and localisation of coronin 2A was studied by immunohistochemistry, immunofluorescence imaging, cell fractionation, and immunoblotting. Functional roles of coronin 2A were analysed by over-expression and knock-down of the protein. Protein interactions were studied by co-immunoprecipitation and pull-down experiments, mass spectrometry analyses, and in vitro kinase and methylation assays.

resultsHistopathological investigation revealed that the expression of coronin 2A in colon tumour cells is up-regulated during the adenoma-adenocarcinoma progression. At the subcellular level, coronin 2A localised to multiple compartments, i.e. F-actin stress fibres, the front of lamellipodia, focal adhesions, and the nuclei. Over-expression of coronin 2A led to a reduction of F-actin stress fibres and elevated cell migration velocity. We identified two novel direct coronin 2A interaction partners. The interaction of coronin 2A with MAPK14 (mitogen activated protein kinase 14 or MAP kinase p38α) led to phosphorylation of coronin 2A and also to activation of the MAPK14 pathway. Moreover, coronin 2A interacted with PRMT5 (protein arginine N-methyltransferase 5), which modulates the sensitivity of tumour cells to TRAIL-induced cell death.

conclusionsWe show that increased expression of coronin 2A is associated with the malignant phenotype of human colon carcinoma. Moreover, we linked coronin 2A to MAPK14 and PRMT5 signalling pathways involved in tumour progression.

Indexed as

Gene Expression Regulation, NeoplasticSignal TransductionAdenocarcinomaAdenomaCell Line, TumorCell MovementColonic NeoplasmsColorectal NeoplasmsHumansMicrofilament ProteinsMitogen-Activated Protein Kinase 14PhosphorylationProtein-Arginine N-MethyltransferasesProtein TransportPseudopodiaReceptors, TNF-Related Apoptosis-Inducing Ligandcoronin proteinsMicrofilament ProteinsMitogen-Activated Protein Kinase 14PRMT5 protein, humanProtein-Arginine N-MethyltransferasesReceptors, TNF-Related Apoptosis-Inducing Ligand

Identifiers

PMID26373535
PMCPMC4612562
OpenAlexW1907207560

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.