ArticleThe Plant cell2015
WRKY Transcription Factors Phosphorylated by MAPK Regulate a Plant Immune NADPH Oxidase in Nicotiana benthamiana.
Article in The Plant cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 144 papers.
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Who cites it
144 citing papers in PubMed, 325 citations in OpenAlex.
- SOBIR1 integrates pathogen recognition and hormonal metabolism in Nicotiana tabacum.Plant physiology · 2026Article
- Transcription factor MdLBD41 negatively regulates resistance to Glomerella leaf spot in apple by suppressing ROS production.The New phytologist · 2026Article
- AtMPK1/AtMPK2 Interact with AtVQ25 to Enhance Its Protein Stability and Coordinately Regulate Salicylic Acid-Mediated Leaf Senescence.Plants (Basel, Switzerland) · 2026Article
- MYB Factors: Hubs of Plant Stress and Hormone Crosstalk.Plant biotechnology journal · 2026Review
- Decoding MAPK cascades in plant immunity: Activation, regulation, integration, and pathogen manipulation.Journal of integrative plant biology · 2026Review
- High Expression of CpMAPK9 Increased Jasmonic Acid Levels Potentially Improving Root Rot Resistance inGenes · 2026Article
- Genome-wide identification of the WRKY gene family in apricot (Prunus armeniaca L.) and functional characterization of PaWRKY57 in salt stress tolerance.BMC plant biology · 2026Article
- ZmWRKY29 Transcriptionally Represses ZmRBOHC to Attenuate ROS Production and Facilitates Gibberella Stalk Rot Susceptibility in Maize.Plant biotechnology journal · 2026Article
- Current Understanding of Cell-Surface Immune Receptors for MAMPs and Plant Parasites in the Solanaceae Family.Molecular plant pathology · 2026Review
- Interplay of different signaling mechanisms in regulating programmed cell death during plant development and in response to stresses.Protoplasma · 2026Review
- The maize mitogen-activated protein kinase kinase kinase gene ZmMAPKKK45 is associated with multiple disease resistance.The New phytologist · 2026Article
- Flagellin-derived peptide flg22 from non-pathogenic Pseudomonas fragi Sneb1990 triggers plant immunity and reduces Meloidogyne incognita infestation.BMC plant biology · 2026Article
- Molecular changes in agroinfiltrated leaves of Nicotiana benthamiana expressing suppressor of silencing P19 and coronavirus-like particles.Plant biotechnology journal · 2026Article
- Visualization and Quantification of Apoplastic ROS by Plant Immune NADPH Oxidase Utilizing Chemiluminescence.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Heterologous expression of Arabidopsis FLS2 in Nicotiana benthamiana confers responsiveness to Ralstonia flg22 and activation of anti-bacterial immunity.Plant biotechnology journal · 2025Article
- Regulatory balance between ear rot resistance and grain yield and their breeding applications in maize and other crops.Journal of advanced research · 2025Review
- Whole-transcriptome sequencing reveals the global molecular responses and ceRNA regulatory network involved in programmed cell death of rice cultivars zyk639 and zyk-lm.BMC genomics · 2025Article
- A phosphorylation-driven ubiquitination switch fine-tunes Alfin-like 7-induced ROS signaling in plant immunity.Science advances · 2025Article
- Principles of signal integration in combinatorial stress acclimatization.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- Ferroptosis in plant immunity.Plant communications · 2025Review
84 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pathogen attack sequentially confers pattern-triggered immunity (PTI) and effector-triggered immunity (ETI) after sensing of pathogen patterns and effectors by plant immune receptors, respectively. Reactive oxygen species (ROS) play pivotal roles in PTI and ETI as signaling molecules. Nicotiana benthamiana RBOHB, an NADPH oxidase, is responsible for both the transient PTI ROS burst and the robust ETI ROS burst. Here, we show that RBOHB transactivation mediated by MAPK contributes to R3a/AVR3a-triggered ETI (AVR3a-ETI) ROS burst. RBOHB is markedly induced during the ETI and INF1-triggered PTI (INF1-PTI), but not flg22-tiggered PTI (flg22-PTI). We found that the RBOHB promoter contains a functional W-box in the R3a/AVR3a and INF1 signal-responsive cis-element. Ectopic expression of four phospho-mimicking mutants of WRKY transcription factors, which are MAPK substrates, induced RBOHB, and yeast one-hybrid analysis indicated that these mutants bind to the cis-element. Chromatin immunoprecipitation assays indicated direct binding of the WRKY to the cis-element in plants. Silencing of multiple WRKY genes compromised the upregulation of RBOHB, resulting in impairment of AVR3a-ETI and INF1-PTI ROS bursts, but not the flg22-PTI ROS burst. These results suggest that the MAPK-WRKY pathway is required for AVR3a-ETI and INF1-PTI ROS bursts by activation of RBOHB.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.