ArticleScientific reports2015
Standardization of the antibody-dependent respiratory burst assay with human neutrophils and Plasmodium falciparum malaria.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 26 citations in OpenAlex.
- Demonstration of the Blood-Stage Plasmodium falciparum Controlled Human Malaria Infection Model to Assess Efficacy of the P. falciparum Apical Membrane Antigen 1 Vaccine, FMP2.1/AS01.The Journal of infectious diseases · 2016Trial
- Vaccine-induced mouse antibodies targeting Plasmodium falciparum PfVFT antigen inhibit blood stages through multiple mechanisms.NPJ vaccines · 2026Article
- Breadth of Fc-mediated effector function correlates with clinical immunity following human malaria challenge.Immunity · 2024Article
- How to Accelerate Early Stage of Malaria Vaccine Development by Optimizing Functional Assays.Vaccines · 2024Review
- Antibody-Dependent Respiratory Burst againstVaccines · 2024Article
- Bi-isotype immunoglobulins enhance antibody-mediated neutrophil activity againstFrontiers in immunology · 2024Article
- Improvement of the antibody-dependent respiratory burst assay for assessing protective immune responses to malaria.Open biology · 2022Article
- Neutrophils in malaria: A double-edged sword role.Frontiers in immunology · 2022Review
- Common virulence gene expression in adult first-time infected malaria patients and severe cases.eLife · 2021Article
- A versatile high-throughput assay to characterize antibody-mediated neutrophil phagocytosis.Journal of immunological methods · 2019Article
- Molecular and Functional Characterization of Fcγ Receptor IIIb-Ligand Interaction: Implications for Neutrophil-Mediated Immune Mechanisms in Malaria.Infection and immunity · 2018Article
- Prospects from systems serology research.Immunology · 2018Review
- Cord blood IgG and the risk of severe Plasmodium falciparum malaria in the first year of life.International journal for parasitology · 2017Article
- Malaria vaccines and human immune responses.Current opinion in microbiology · 2016Review
- Progress and prospects for blood-stage malaria vaccines.Expert review of vaccines · 2016Review
- Targets and Mechanisms Associated with Protection from Severe Plasmodium falciparum Malaria in Kenyan Children.Infection and immunity · 2016Article
- Changes in Serological Immunology Measures in UK and Kenyan Adults Post-controlled Human Malaria Infection.Frontiers in microbiology · 2016Article
Corrections and comments
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Authors and funding
14 authors at 7 institutions in 4 countries.
Funding
Abstract
The assessment of naturally-acquired and vaccine-induced immunity to blood-stage Plasmodium falciparum malaria is of long-standing interest. However, the field has suffered from a paucity of in vitro assays that reproducibly measure the anti-parasitic activity induced by antibodies in conjunction with immune cells. Here we optimize the antibody-dependent respiratory burst (ADRB) assay, which assesses the ability of antibodies to activate the release of reactive oxygen species from human neutrophils in response to P. falciparum blood-stage parasites. We focus particularly on assay parameters affecting serum preparation and concentration, and importantly assess reproducibility. Our standardized protocol involves testing each serum sample in singlicate with three independent neutrophil donors, and indexing responses against a standard positive control of pooled hyper-immune Kenyan sera. The protocol can be used to quickly screen large cohorts of samples from individuals enrolled in immuno-epidemiological studies or clinical vaccine trials, and requires only 6 μL of serum per sample. Using a cohort of 86 samples, we show that malaria-exposed individuals induce higher ADRB activity than malaria-naïve individuals. The development of the ADRB assay complements the use of cell-independent assays in blood-stage malaria, such as the assay of growth inhibitory activity, and provides an important standardized cell-based assay in the field.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.