ArticleBiomaterials science2015
Protein-engineered scaffolds for in vitro 3D culture of primary adult intestinal organoids.
Article in Biomaterials science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed.
- A Fully Defined GelMA-Based Matrix Allows Fine Tuning of Tissue-Relevant Biomechanical and Biochemical Cues for Organoid Culture.Advanced healthcare materials · 2026Article
- AnBiofabrication · 2025Article
- Mechanically and Chemically Defined PEG Hydrogels Improve Reproducibility in Human Cardioid Development.Advanced healthcare materials · 2025Article
- Engineering CAR-T Therapeutics for Enhanced Solid Tumor Targeting.Advanced materials (Deerfield Beach, Fla.) · 2025Review
- Reproducible extracellular matrices for tumor organoid culture: challenges and opportunities.Journal of translational medicine · 2025Review
- The Use of Gut Organoids: To Study the Physiology and Disease of the Gut Microbiota.Journal of cellular and molecular medicine · 2025Review
- Developments in gastrointestinal organoid cultures to recapitulate tissue environments.Frontiers in bioengineering and biotechnology · 2025Review
- Organoid scaffold materials: research and application.Frontiers in bioengineering and biotechnology · 2025Review
- Polymer design of microwell hydrogels influences epithelial-mesenchymal interactions during human bronchosphere formation.Advanced nanobiomed research · 2025Article
- Pros and Cons of Human Brain Organoids to Study Alzheimer's Disease.Aging and disease · 2024Review
- Efficient Generation of Skin Organoids from Pluripotent Cells via Defined Extracellular Matrix Cues and Morphogen Gradients in a Spindle-Shaped Microfluidic Device.Advanced healthcare materials · 2024Article
- Listen to Your Gut: Key Concepts for Bioengineering Advanced Models of the Intestine.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Toward reproducible tumor organoid culture: focusing on primary liver cancer.Frontiers in immunology · 2024Review
- Article
- Patient-derived organoid culture in epithelial ovarian cancers-Techniques, applications, and future perspectives.Cancer medicine · 2023Review
- Engineered biomaterials in stem cell-based regenerative medicine.Life medicine · 2023Review
- Accelerating aging with dynamic biomaterials: Recapitulating aged tissue phenotypes in engineered platforms.iScience · 2023Review
- A matrigel-free method for culture of pancreatic endocrine-like cells in defined protein-based hydrogels.Frontiers in bioengineering and biotechnology · 2023Article
- Tuning Polymer Hydrophilicity to Regulate Gel Mechanics and Encapsulated Cell Morphology.Advanced healthcare materials · 2022Article
- Human organoids in basic research and clinical applications.Signal transduction and targeted therapy · 2022Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Though in vitro culture of primary intestinal organoids has gained significant momentum in recent years, little has been done to investigate the impact of microenvironmental cues provided by the encapsulating matrix on the growth and development of these fragile cultures. In this work, the impact of various in vitro culture parameters on primary adult murine organoid formation and growth are analyzed with a focus on matrix properties and geometric culture configuration. The air-liquid interface culture configuration was found to result in enhanced organoid formation relative to a traditional submerged configuration. Additionally, through use of a recombinantly engineered extracellular matrix (eECM), the effects of biochemical and biomechanical cues were independently studied. Decreasing mechanical stiffness and increasing cell adhesivity were found to increase organoid yield. Tuning of eECM properties was used to obtain organoid formation efficiency values identical to those observed in naturally harvested collagen I matrices but within a stiffer construct with improved ease of physical manipulation. Increased ability to remodel the surrounding matrix through mechanical or enzymatic means was also shown to enhance organoid formation. As the engineering and tunability of recombinant matrices is essentially limitless, continued property optimization may result in further improved matrix performance and may help to identify additional microenvironmental cues that directly impact organoid formation, development, differentiation, and functional behavior. Continued culture of primary organoids in recombinant matrices could therefore prove to be largely advantageous in the field of intestinal tissue engineering for applications in regenerative medicine and in vitro tissue mimics.
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Registered trials
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